ANTI-DIABETIC STUDY AND ITS QUANTITATIVE REAL-TIME PCR ANALYSIS OF THE INSR GENE IN SILVERNANOPARTICLE EXTRACT OF CHAMAECOSTUS CUSPIDATUS

Manufacturing metal oxide nanoparticles for chemical and biomedical applications is made possible by green synthesis, an eco-friendly approach. This investigation examined how silver nanoparticle leaf extract affected gene expression and its ability to treat diabetes. In this study, the Anti-diabetic study was evaluated by following the Glucose utilization control method. Furthermore, the study continues with Quantitative Real Time-PCR Analysis of the INSR Gene. The analysis's findings showed that, when used as a control, metformin exhibits a glucose percentage of 169.26, whereas silver nanoparticle extracts exhibit a percentage control of 169.53. HepG2 cells treated with AgNPs C.cuspidtus showed upregulated INSR gene expression, going from 1-fold in control cells to 1.51-fold in treated cells. The relative gene expression of the control is typically set to 1 when compared to the sample. The study shows that glucose absorption in AgNPs extract is slightly higher than in methanolic extract. As a result, there is a pressing mandate to protect this species and investigate its biological applications to achieve more significant and worthwhile outcomes.