广宿主范围阪崎克罗诺杆菌噬菌体的分离、鉴定及其在乳制品中的生物防治潜力

IF 4.6 3区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Quality Assurance and Safety of Crops & Foods Pub Date : 2021-08-06 DOI:10.15586/qas.v13i3.890

Li Huaxiang, Yang Xiaojun, Xiao-yan Zhu, Lu Gao, Sheng-qi Rao, Lei Yuan, Zhen-quan Yang

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引用次数: 11

摘要

阪崎克罗诺杆菌（C.sakazakii）是一种污染乳制品（如奶粉）的重要病原体，可导致婴儿高死亡率。噬菌体作为一种潜在的生物防治剂，是控制乳制品生产及其环境中这种病原体的一种很好的替代方法。因此，重要的是通过分离和表征针对阪崎肠杆菌的广泛宿主范围的噬菌体来完成阪崎大肠杆菌噬菌体文库，以供对照使用。本研究以不同来源的阪崎肠杆菌菌株为宿主，采用双层平板法从人类粪便和污水样品中分离纯化噬菌体。然后对这些噬菌体的生物学特性、抗菌特性和基因组进行了研究。最后，分离出10个感染阪崎肠杆菌的毒力噬菌体（EspYZU01–EspYZU10），并鉴定为属于Myoviride、Podoviridae、Tectivirus和Styloviride科。EspYZU08噬菌体的寄主范围最广，可感染阪崎肠杆菌的5个寄主菌株。所有10个噬菌体在50°C和pH 5-9时都保持了感染性。EspYZU05和EspYZU_8的基因组均为双链DNA，大小分别为41723bp和145582bp，G+C含量分别为55.69%和46.75%，开放阅读框分别为47和103。在EspYZU05和EspYZU18中均未检测到毒素和抗生素抗性基因。在4°C、25°C和37°C的液体肉汤和牛奶中，噬菌体EspYZU08和噬菌体cocktail-3（EspYZU11+EspYZU23+EspYZU48+EspYZU9+EspYZU10）对阪崎肠杆菌表现出优异的抗菌效果，表明本研究中的噬菌体在开发乳制品及其加工环境中的阪崎大肠杆菌生物防治剂方面具有巨大的潜力。

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Isolation and characterization of broad host-range of bacteriophages infecting Cronobacter sakazakii and its biocontrol potential in dairy products

Cronobacter sakazakii (C. sakazakii) is an important pathogen contaminating dairy products (e.g., milk pow-der) and causes high mortality in infants. Bacteriophage as a potential biocontrol agent is a good alternative method for the control of this pathogen in dairy production and its environment. Thus, it is important to complete the C. sakazakii phage library by isolating and characterizing the broad host range of bacteriophage against C. sakazakii for control use. In this study, C. sakazakii strains from different sources were used as hosts to isolate and purify phages from human stool and sewage samples by double-layer plates. The biological characteristics, antibacterial properties, and genomes of these phages were then studied. Finally, ten virulent phages (EspYZU01–EspYZU10) infecting C. sakazakii were isolated and identified as belonging to the Myoviridae, Podoviridae, Tectivirus, and Stylovinidae families. Phage EspYZU08 presented the broadest host range and could infect all the five host strains of C. sakazakii. All 10 phages retained their infectivity at 50°C and pH 5–9. Both genomes of EspYZU05 and EspYZU08 were double-stranded DNAs with sizes of 41723 bp and 145582 bp, G+C contents of 55.69% and 46.75%, and open reading frames of 47 and 103, respectively. No toxins and antibiotic resistance genes were detected in both EspYZU05 and EspYZU08. Phage EspYZU08 and phage cocktail-3 (EspYZU01 + EspYZU03 + EspYZU08 + EspYZU09 + EspYZU10) presented excellent antibacterial efficacy for C. sakazakii in liquid broth and milk at 4°C, 25°C, and 37°C, suggesting that the phages in this study have great potential for the development of biocontrol agents against C. sakazakii in dairy and its processing environment.

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来源期刊

Quality Assurance and Safety of Crops & Foods FOOD SCIENCE & TECHNOLOGY-

CiteScore

4.60

自引率

7.50%

发文量

审稿时长

1 months

期刊介绍： ''Quality Assurance and Safety of Crops & Foods'' is an international peer-reviewed journal publishing research and review papers associated with the quality and safety of food and food sources including cereals, grains, oilseeds, fruits, root crops and animal sources. It targets both primary materials and their conversion to human foods. There is a strong focus on the development and application of new analytical tools and their potential for quality assessment, assurance, control and safety. The scope includes issues of risk assessment, traceability, authenticity, food security and socio-economic impacts. Manuscripts presenting novel data and information that are likely to significantly contribute to scientific knowledge in areas of food quality and safety will be considered. ''Quality Assurance and Safety of Crops & Foods'' provides a forum for all those working in the specialist field of food quality and safety to report on the progress and outcomes of their research.