禽分枝杆菌亚种的分子分型。哥伦比亚安蒂奥基亚奶牛场的副结核

IF 0.4 4区农林科学 Q4 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Revista Mvz Cordoba Pub Date : 2020-08-13 DOI:10.21897/rmvz.1816

N. M. Correa-Valencia, Roberto Damián Moyano, M. Romano, J. A. Fernández-Silva

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引用次数: 3

摘要

目标。目的:测定禽分枝杆菌亚种。哥伦比亚奶牛群环境样本中的副结核(MAP)分子多样性。材料和方法。选取25头IS900-qPCR map阳性奶牛的环境样品，在含有mycoblin J的Herrold’s蛋黄培养基中重复培养，获得分离菌株。可疑菌落经MAPIS900-qPCR确认。采用分枝杆菌穿插重复单位-可变数目串联重复(MIRU-VNTR)和多位点短序列重复(MLSSR)技术对阳性DNA进行分型，分析分离株间的遗传差异。结果。MIRU-VNTR分型显示两种不同的基因型(INMV 2和INMV 36)。采用MLSSR技术，提高了玉米的抗氧化能力

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Tipificación molecular de Mycobacterium avium subsp. paratuberculosis en lecherías de Antioquia, Colombia

Objective. To determine Mycobacterium avium subsp. paratuberculosis (MAP) molecular diversity in environmental samples from Colombian dairy herds. Materials and methods. Environmental samples from 25 IS900-qPCR MAP-positive dairy herds were cultured by duplicate in Herrold ́s egg yolk medium with mycobactin J to obtain isolates. Suspicious colonies were confirmed by MAPIS900-qPCR. Positive DNA was sub-typed using mycobacterial interspersed repetitive units-variable number of tandem repeat (MIRU-VNTR) and multilocus short sequence repeats (MLSSR) techniques to analyze the genetic differences between the isolates. Results. Sub-typing revealed two different genotypes by MIRU-VNTR (INMV 2 and INMV 36). MLSSR technique was carried out to increase the

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来源期刊

Revista Mvz Cordoba 农林科学-奶制品与动物科学

CiteScore

0.70

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： The Journal MVZ Córdoba is an open access international scientific journal financed and edited by the University of Córdoba (Colombia). The journal publishes quarterly, continuously in PDF, XML, Epub, original articles, literature reviews, brief communications and clinical cases, peer-reviewed (double-blind) in Spanish and English, which are related to the agricultural and veterinary sciences. The journal is directed to natural and legal persons of veterinary medicine, animal husbandry, public health, epidemiology, aquaculture, biology, basic biomedical sciences and biotechnology and constitutes a space for academic and scientific discussion around the work of professionals in Veterinary Medicine and Zootechnics. Four-monthly publication. "The Journal MVZ Córdoba supports the policies for registration of clinical trials of the World Health Organization (WHO) and the International Committee of Medical Journal Editors (ICMJE), since it recognizes the importance of these initiatives for international registration and dissemination. of information about clinical studies, in open access. As a result, since 2007, the journal MVZ Córdoba only publishes clinical research articles that have received an identification number in one of the Clinical Trial Registries validated by the criteria established by WHO and ICMJE, whose addresses are available in the ICMJE website. The identification number is recorded at the end of the summary. "