阿克替隆诱导人乳腺腺癌细胞凋亡

IF 0.9 4区医学 Q4 PHARMACOLOGY & PHARMACY

Bangladesh Journal of Pharmacology Pub Date : 2018-12-14 DOI:10.3329/BJP.V13I4.38026

M. Mosaddegh, Mahboobeh Irani, M. Hamzeloo-Moghadam

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引用次数: 3

摘要

癌症研究人员一直很感兴趣地评估草药提取物的效果，因为这些天然材料可能是寻找新抗癌剂的合适来源。在本研究中，采用MTT法评估了阿替隆甲醇提取物对两种人乳腺癌症细胞系MCF-7和MDA-MB-468的细胞毒性作用。还使用膜联蛋白-V/碘化丙啶测定、Hoechst 33258染色和流式细胞仪评估细胞周期来评估细胞凋亡潜力。MTT结果显示MCF-7和MDA-MB-468细胞的IC50分别为69.2和32.6μg/mL。细胞凋亡测定的结果证实了植物提取物在乳腺癌症细胞系中的细胞凋亡潜力，这表明A.repens可用于进一步的癌症研究。

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Acroptilon repens induces apoptosis in human breast adenocarcinoma

Evaluating the effect of herbal extracts has been always interesting for cancer researchers considering that these natural materials could be suitable sources for finding new anti-cancer agents. In the present study, Acroptilon repens methanol extract had been evaluated for its cytotoxic effects in two human breast cancer cell lines, MCF-7 and MDA-MB-468, using MTT assay. The apoptosis potential had also been evaluated using annexin-V/propidium iodide assay, Hoechst 33258 staining and evaluating the cell cycle with flow cytometery. The MTT results showed cytotoxicity with IC50 values of 69.2 and 32.6 μg/mL for MCF-7 and MDA-MB-468 cells, respectively. The results of the apoptosis assays confirmed the apoptosis potential of the plant extract in the breast cancer cell lines suggesting A. repens for further cancer studies.

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来源期刊

Bangladesh Journal of Pharmacology PHARMACOLOGY & PHARMACY-

CiteScore

1.50

自引率

18.80%

发文量

审稿时长

>12 weeks

期刊介绍： Bangladesh Journal of Pharmacology (Bangladesh J Pharmacol) is an open access, video component, peer-reviewed journal of the Bangladesh Pharmacological Society (BDPS). A scholarly publication is defined ''open access'' when there are no financial, legal or technical barriers to accessing it. Anyone can read, download, copy, distribute, print or use it in educational purpose within the legal agreements. Peer review of a manuscript means the evaluation of research work by one or more people with similar competences. It can be open or blind. We do the single-blind peer review. Our readers want to know the methodology used by the researchers which is very important to get the results. The video clip of one of the methodologies is prepared by the authors and submit it to publish.