新型抗小鼠CCR2单克隆抗体(C2Mab-6)的n端肽免疫制备

Q3 Medicine

Monoclonal Antibodies in Immunodiagnosis and Immunotherapy Pub Date : 2022-04-04 DOI:10.1089/mab.2021.0063

Tomohiro Tanaka, Guanjie Li, Teizo Asano, Masaki Saito, M. Kaneko, Hiroyuki Suzuki, Y. Kato

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引用次数: 12

摘要

CC趋化因子受体2型(CCR2)属于g蛋白偶联受体超家族，在免疫细胞和肿瘤细胞表面表达。CCR2结合CC基元趋化因子2/单核细胞趋化蛋白-1，这是一种CC趋化因子，由多种细胞产生，包括免疫相关细胞和肿瘤。因此，开发针对CCR2的敏感单克隆抗体(mab)已被用于治疗和诊断。本研究建立了一种新型、特异、敏感的抗小鼠CCR2 (mCCR2)单抗;C2Mab-6(大鼠IgG1、kappa)，采用mCCR2合成肽免疫法。C2Mab-6与表达内源性mCCR2的中国仓鼠卵巢k1细胞和L1210(小鼠白血病)细胞发生反应。此外，C2Mab-6对这两种细胞都表现出很高的结合亲和力。因此，C2Mab-6可以作为流式细胞术分析mccr2相关生物反应的有用工具。

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Development of a Novel Anti-Mouse CCR2 Monoclonal Antibody (C2Mab-6) by N-Terminal Peptide Immunization.

The CC chemokine receptor type-2 (CCR2) belongs to the G-protein-coupled receptor superfamily, expressed on the cell surface of immune cells and tumors. CCR2 binds to the CC motif chemokine 2/monocyte chemoattractant protein-1, a CC chemokine, which is produced by various cells, including immune-related cells and tumors. Therefore, the development of sensitive monoclonal antibodies (mAbs) for CCR2 has been desired for treatment and diagnosis. This study established a novel, specific, and sensitive anti-mouse CCR2 (mCCR2) mAb; C2Mab-6 (rat IgG1, kappa), using the mCCR2 synthetic peptide immunization method. C2Mab-6 reacted with mCCR2-overexpressed Chinese hamster ovary-K1 cells and L1210 (murine leukemia) cells, which express endogenous mCCR2 in flow cytometry. Furthermore, C2Mab-6 showed a high binding affinity for both cells. Hence, C2Mab-6 can be a useful tool for analyzing mCCR2-related biological responses, using flow cytometry.

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来源期刊

Monoclonal Antibodies in Immunodiagnosis and Immunotherapy Medicine-Immunology and Allergy

CiteScore

4.80

自引率

0.00%

发文量