血液中醛固酮浓度不同检测方法的一致性

Q4 Medicine

中华内分泌代谢杂志 Pub Date : 2019-11-25 DOI:10.3760/CMA.J.ISSN.1000-6699.2019.11.003

Fengfan Zheng, Ying Song, Shumin Yang, Jinbo Hu, Wenwen He, Zhihong Wang, Q. Cheng

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引用次数: 1

摘要

目的评价不同检测血中醛固酮浓度方法的一致性，建立液相色谱-串联质谱(LC-MS/MS)测定血清醛固酮浓度的参考区间。方法对138例健康成人、67例原发性高血压患者和23例原发性醛固酮增多症患者，采用LC-MS/MS、化学发光法(Diasorin、国产A、B系统)和放射免疫法(RIA)测定血醛固酮浓度。结果CLIA(Domestic B)法测定的醛固酮浓度与LC-MS/MS法测定的醛固酮浓度差异无统计学意义(P 0.05)， RIA法测定的醛固酮浓度与LC-MS/MS法测定的醛固酮浓度差异无统计学意义(P<0.01);Bland-Altman分析表明，醛固酮浓度测定方法的一致性较差。LC-MS/MS测定血清醛固酮浓度的参考区间为15.2 ~ 222.2 pg/ml。结论不同方法测定的血醛固酮浓度有显著性差异。临床上，使用不同的方法可能需要一个特定的参考区间。关键词:醛固酮;液相色谱-串联质谱法;化学发光分析;放射免疫检定法

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On the consistency of different methods for detecting aldosterone concentration in blood

Objective To evaluate the consistency of different methods for detecting aldosterone concentration in blood and to establish a reference interval of serum aldosterone concentration by liquid chromatography-tandem mass spectrometry(LC-MS/MS). Methods Concentrations of blood aldosterone were measured by LC-MS/MS, chemiluminescent assays (Diasorin, Domestic A and B systems) and radioimmunoassay (RIA) in 138 healthy adults, 67 patients with essential hypertension and 23 patients with primary aldosteronism. Results Aldosterone concentrations measured by various methods were quiet different(P 0.05) between CLIA(Domestic B) and LC-MS/MS, 0.338(P<0.01) as well as between RIA and LC-MS/MS. Bland-Altman analysis for the measurements showed poor consistency among methods for aldosterone concentrations. The reference interval was 15.2-222.2 pg/ml for serum aldosterone concentrations by LC-MS/MS. Conclusions There are significant differences of blood aldosterone concentrations determined by different methods. Clinically, a specified reference interval might be needed while using different methods. Key words: Aldosterone; Liquid chromatography-tandem mass spectrometry; Chemiluminescent assays; Radioimmunoassay

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来源期刊

中华内分泌代谢杂志 Medicine-Endocrinology, Diabetes and Metabolism

CiteScore

0.60

自引率

0.00%

发文量

7243

期刊介绍： The Chinese Journal of Endocrinology and Metabolism was founded in July 1985. It is a senior academic journal in the field of endocrinology and metabolism sponsored by the Chinese Medical Association. The journal aims to be the "Chinese broadcaster of new knowledge on endocrinology and metabolism worldwide". It reports leading scientific research results and clinical diagnosis and treatment experience in endocrinology and metabolism and related fields, as well as basic theoretical research that has a guiding role in endocrinology and metabolism clinics and is closely integrated with clinics. The journal is a core journal of Chinese science and technology (a statistical source journal of Chinese science and technology papers), and is included in Chinese and foreign statistical source journal databases such as the Chinese Science and Technology Papers and Citation Database, Chemical Abstracts, and Scopus.