巴西圣保罗大西洋雨林中朱氏Stigonema jureiensis sp.nov.（Nostocales，Cyanobacteria）的形态和分子特征

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2019-10-30 DOI:10.5507/FOT.2019.009

Guilherme S. Hentschke, J. Rigonato, D. B. Genuário, H. D. Laughinghouse, C. L. Sant’Anna

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引用次数: 2

摘要

热带地区被认为是生物多样性的热点，Stigonema属在世界各地的开发不足，主要是由于培养的困难。在本研究中，我们对热带大西洋雨林（巴西圣保罗）的一个天然Stigonema种群的16S rRNA基因进行了测序，并根据系统发育和形态学数据描述了S.jureiensis sp.nov。为了进行分子分析，从种群的等分试样中分离出基因组DNA，这些等分试样之前在显微镜下使用锥形巴斯德移液管手动清洁，以去除其他生物体和碎片。为了进行形态学分析，使用显微镜对30个个体进行分析和拍照。最大似然和贝叶斯系统发育都支持朱雷S.jureiensis作为一个新物种，形态学分析揭示了一种特殊类型的镶嵌华丽鞘的存在。

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Morphological and molecular characterization of Stigonema jureiensis sp. nov. (Nostocales, Cyanobacteria) from the Atlantic Rainforest, São Paulo, Brazil

Tropical regions are considered hotspots of biodiversity, and the genus Stigonema has been underexplored in all world, mainly due to the difficulties of culturing. In this study, we sequenced the 16S rRNA gene of a natural Stigonema population from the tropical Atlantic Rainforest (Sao Paulo, Brazil) and describe S. jureiensis sp. nov. based on phylogenetic and morphological data. For the molecular analysis, the genomic DNA was isolated from aliquots of the population, which were previously manually cleaned under a microscope, using a point-tapered Pasteur pipette to remove other organisms and debris. For morphological analysis, 30 individuals were analyzed and photographed using microscope. Both maximum likelihood and Bayesian phylogenies support S. jureiensis as a new species, and morphological analysis revealed the presence of a special type of mosaic ornate sheaths.

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.