西洋参细胞培养液中人参皂苷和酚类化合物及其抗氧化性能

IF 1.1 4区生物学 Q3 PLANT SCIENCES

Acta Societatis Botanicorum Poloniae Pub Date : 2019-12-10 DOI:10.5586/asbp.3638

E. Kochan, G. Szymańska, Izabela Grzegorczy-Karolak, P. Szymczyk, Monika Sienkiewicz

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引用次数: 4

摘要

对西洋参愈伤组织和悬浮培养液的抗氧化性能进行了研究。并测定了各培养物的人参皂苷含量和总酚含量。通过HPLC分析，9种皂苷在红愈伤组织(RCL)、绿愈伤组织(GCL)和悬浮培养中的总含量分别为2.08、1.69和0.202 mg g−1干重。采用Folin-Ciocalteu法估算TPC。悬浮培养提取物的TPC分别比RCL和GCL高36.7%和17.6%。采用体外ABTS[2,2'-氮基-双(3-乙基苯并噻唑啉-6-磺酸)]和FRAP(铁还原抗氧化能力)法评价提取物的抗氧化活性;甲醇悬浮液提取物的抗氧化活性高于愈伤组织提取物。

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Ginsenoside and phenolic compounds in hydromethanolic extracts of American ginseng cell cultures and their antioxidant properties

The present study was performed to investigate the antioxidant properties of callus and suspension culture extracts of Panax quinquefolium (American ginseng). The ginsenoside content and the total phenolic content (TPC) in these cultures were also examined. The total amount of the nine studied saponins was found to be 2.08, 1.69, and 0.202 mg g −1 dry weight in red callus line (RCL), green callus line (GCL), and suspension cultures, respectively, by HPLC analysis. The TPC was estimated using the Folin–Ciocalteu method. The TPC of the suspension culture extracts was approximately 36.7% and 17.6% higher than that of the RCL and GCL, respectively. The antioxidant activity of the extracts was evaluated using the in vitro ABTS [2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid)] and FRAP (ferric reducing antioxidant power) assays; the methanolic P. quinquefolium suspension culture extracts demonstrated stronger antioxidant activity than that of the callus cultures.

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来源期刊

Acta Societatis Botanicorum Poloniae 生物-植物科学

CiteScore

2.00

自引率

10.00%

发文量

审稿时长

1 months

期刊介绍： The journal has been published since 1923 and offers Open Access publication of original research papers, short communications, and reviews in all areas of plant science, including evolution, ecology, genetics, plant structure and development, physiology and biochemistry.