聚合酶链式反应检测印度泰米尔纳德邦水牛(Bubalus bubalis)中的泰勒氏菌和无浆体菌

IF 0.2 4区 农林科学 Q4 AGRICULTURE, DAIRY & ANIMAL SCIENCE
G. Ponnudurai, N. Rani, B. Rubinibala, Alagarsamy Alagesan, R. Saravanan
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引用次数: 0

摘要

从泰米尔纳德邦三个农业气候区(即Cauvery delta、西北和南部地区)的水牛身上采集了150份肝素化血液样本和相同数量的血液涂片,通过血液涂片检查和聚合酶链式反应检测泰勒氏症和无浆血症。血液涂片检查显示,没有发现任何动物携带任何血原生动物寄生虫。而PCR在西北区和Cauvery三角洲分别检测到26.9%和48.0%的动物和10%和15%的动物的泰勒虫和无浆虫,而在南区没有检测到任何原生动物阳性。此外,8只(5.3%)动物出现泰勒氏菌和无浆体混合感染。为确定与血原生动物寄生虫发生相关的危险因素而收集的数据分析显示,纯种Murrah的泰勒氏体和无浆体阳性率(30.7%和46.1%)高于非描述性品种(8.7%和16.0%),其次是2岁和>4岁。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Detection of Theileria and Anaplasma spp. in carrier buffaloes (Bubalus bubalis) by polymerase chain reaction in Tamil Nadu, India
A total of 150 heparinised blood samples and equal number of blood smears were collected from buffaloes in three agro-climatic zones of Tamil Nadu viz., Cauvery delta, North - western and Southern zones to detect Theileria and Anaplasma by blood smear examination and Polymerase Chain Reaction. The blood smear examination revealed that none of animals were found to harbour any haemoprotozoan parasites. Whereas PCR detected Theileria and Anaplasma in 26.9% and 48.0%, and 10% and 15% of the animals, respectively in north- western zone and Cauvery delta, however no animal from south zone was detected positive for any protozoan. Besides, mixed infection of Theileria and Anaplasma was recorded in 8 (5.3%) animals. The analysis of data collected to determine the risk factors associated with occurrence of haemoprotozoan parasites revealed that the pure breed Murrah showed higher percentage of Theileria and Anaplasma positivity (30.7% and 46.1%) than non - descriptive breed (8.7% and 16.0%). The percentage of positivity of haemoprotozoan parasites was found to be higher among animals of 1 year, followed by 2 years and > 4 years of age.
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来源期刊
Buffalo Bulletin
Buffalo Bulletin AGRICULTURE, DAIRY & ANIMAL SCIENCE-
CiteScore
0.50
自引率
0.00%
发文量
53
审稿时长
>12 weeks
期刊介绍: Buffalo Bulletin is published quarterly in January-March, April-June, July-September and October-December. Contributions on any aspect of research or development, progress reports of projects and news on buffalo will be considered for publication in the bulletin.
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