Nahideh Nazdikbin Yamchi, Farhad Amjadi, Rahim Beheshti, Mehdi Hassanpour, Reza Shirazi, Amin Tamadon, Reza Rahbarghazi, Mahdi Mahdipour
{"title":"骨髓CD144+内皮细胞与CD146+间充质干细胞治疗POF大鼠的疗效比较","authors":"Nahideh Nazdikbin Yamchi, Farhad Amjadi, Rahim Beheshti, Mehdi Hassanpour, Reza Shirazi, Amin Tamadon, Reza Rahbarghazi, Mahdi Mahdipour","doi":"10.34172/bi.2023.27781","DOIUrl":null,"url":null,"abstract":"<p><p></p><p><strong>Introduction: </strong>Premature ovarian insufficiency (POI) is a challenging issue in terms of reproduction biology. In this study, therapeutic properties of bone marrow CD146<sup>+</sup> mesenchymal stem cells (MSCs) and CD144<sup>+</sup> endothelial cells (ECs) were separately investigated in rats with POI.</p><p><strong>Methods: </strong>POI rats were classified into control POI, POI + CD146<sup>+</sup> MSCs, and POI + CD144<sup>+</sup> ECs groups. Enriched CD146<sup>+</sup> MSCs and CD144<sup>+</sup> ECs were directly injected into ovarian tissue (15 × 10<sup>4</sup> cells/10 μL) in relevant groups. After 4 weeks, follicle-stimulating hormone (FSH), luteinizing hormone (LH), and estradiol (E<sub>2</sub>) levels were measured in blood samples. Ovarian tissues were collected and subjected to Hematoxylin-Eosin and Masson's trichrome staining. The expression of <i>angp-2, vegfr-2, smad-2, -4, -6,</i> and <i>tgf-β1</i> was studied using qRT-PCR analysis. Histopathological examination indicated an increased pattern of atretic follicles in the POI group related to the control rats (<i>P</i><0.0001).</p><p><strong>Results: </strong>Data indicated that injection of POI + CD146<sup>+</sup> MSCs and CD144<sup>+</sup> ECs in POI rats reduced atretic follicles and increased the number of normal follicles (<i>P</i><0.01). Along with these changes, the content of blue-colored collagen fibers was diminished after cell transplantation. Besides, cell transplantation in POI rats had the potential to reduce increased FSH, and LH levels (<i>P</i><0.05). In contrast, E2 content was increased in POI + CD146<sup>+</sup> MSCs and POI + CD144<sup>+</sup> ECs groups compared to control POI rats, indicating restoration of follicular function. CD144<sup>+</sup> (<i>smad-2</i>, and <i>-4</i>) and CD146<sup>+</sup> (<i>smad-6</i>) cells altered the activity of genes belonging TGF-β signaling pathway. Unlike POI + CD146<sup>+</sup> MSCs, aberrant angiogenesis properties were significantly down-regulated in POI + CD144<sup>+</sup> ECs related to the control POI group (<i>P</i><0.05).</p><p><strong>Conclusion: </strong>The transplantation of bone marrow CD146<sup>+</sup> and CD144<sup>+</sup> cells can lead to the restoration of ovarian tissue function in POI rats via modulating different mechanisms associated with angiogenesis and fibrosis.</p>","PeriodicalId":48614,"journal":{"name":"Bioimpacts","volume":null,"pages":null},"PeriodicalIF":2.2000,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10676523/pdf/","citationCount":"0","resultStr":"{\"title\":\"Comparison the therapeutic effects of bone marrow CD144<sup>+</sup> endothelial cells and CD146<sup>+</sup> mesenchymal stem cells in POF rats.\",\"authors\":\"Nahideh Nazdikbin Yamchi, Farhad Amjadi, Rahim Beheshti, Mehdi Hassanpour, Reza Shirazi, Amin Tamadon, Reza Rahbarghazi, Mahdi Mahdipour\",\"doi\":\"10.34172/bi.2023.27781\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p></p><p><strong>Introduction: </strong>Premature ovarian insufficiency (POI) is a challenging issue in terms of reproduction biology. In this study, therapeutic properties of bone marrow CD146<sup>+</sup> mesenchymal stem cells (MSCs) and CD144<sup>+</sup> endothelial cells (ECs) were separately investigated in rats with POI.</p><p><strong>Methods: </strong>POI rats were classified into control POI, POI + CD146<sup>+</sup> MSCs, and POI + CD144<sup>+</sup> ECs groups. Enriched CD146<sup>+</sup> MSCs and CD144<sup>+</sup> ECs were directly injected into ovarian tissue (15 × 10<sup>4</sup> cells/10 μL) in relevant groups. After 4 weeks, follicle-stimulating hormone (FSH), luteinizing hormone (LH), and estradiol (E<sub>2</sub>) levels were measured in blood samples. Ovarian tissues were collected and subjected to Hematoxylin-Eosin and Masson's trichrome staining. The expression of <i>angp-2, vegfr-2, smad-2, -4, -6,</i> and <i>tgf-β1</i> was studied using qRT-PCR analysis. Histopathological examination indicated an increased pattern of atretic follicles in the POI group related to the control rats (<i>P</i><0.0001).</p><p><strong>Results: </strong>Data indicated that injection of POI + CD146<sup>+</sup> MSCs and CD144<sup>+</sup> ECs in POI rats reduced atretic follicles and increased the number of normal follicles (<i>P</i><0.01). Along with these changes, the content of blue-colored collagen fibers was diminished after cell transplantation. Besides, cell transplantation in POI rats had the potential to reduce increased FSH, and LH levels (<i>P</i><0.05). In contrast, E2 content was increased in POI + CD146<sup>+</sup> MSCs and POI + CD144<sup>+</sup> ECs groups compared to control POI rats, indicating restoration of follicular function. CD144<sup>+</sup> (<i>smad-2</i>, and <i>-4</i>) and CD146<sup>+</sup> (<i>smad-6</i>) cells altered the activity of genes belonging TGF-β signaling pathway. Unlike POI + CD146<sup>+</sup> MSCs, aberrant angiogenesis properties were significantly down-regulated in POI + CD144<sup>+</sup> ECs related to the control POI group (<i>P</i><0.05).</p><p><strong>Conclusion: </strong>The transplantation of bone marrow CD146<sup>+</sup> and CD144<sup>+</sup> cells can lead to the restoration of ovarian tissue function in POI rats via modulating different mechanisms associated with angiogenesis and fibrosis.</p>\",\"PeriodicalId\":48614,\"journal\":{\"name\":\"Bioimpacts\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.2000,\"publicationDate\":\"2023-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10676523/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Bioimpacts\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://doi.org/10.34172/bi.2023.27781\",\"RegionNum\":4,\"RegionCategory\":\"工程技术\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2023/7/29 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q3\",\"JCRName\":\"PHARMACOLOGY & PHARMACY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bioimpacts","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.34172/bi.2023.27781","RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2023/7/29 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"PHARMACOLOGY & PHARMACY","Score":null,"Total":0}
Comparison the therapeutic effects of bone marrow CD144+ endothelial cells and CD146+ mesenchymal stem cells in POF rats.
Introduction: Premature ovarian insufficiency (POI) is a challenging issue in terms of reproduction biology. In this study, therapeutic properties of bone marrow CD146+ mesenchymal stem cells (MSCs) and CD144+ endothelial cells (ECs) were separately investigated in rats with POI.
Methods: POI rats were classified into control POI, POI + CD146+ MSCs, and POI + CD144+ ECs groups. Enriched CD146+ MSCs and CD144+ ECs were directly injected into ovarian tissue (15 × 104 cells/10 μL) in relevant groups. After 4 weeks, follicle-stimulating hormone (FSH), luteinizing hormone (LH), and estradiol (E2) levels were measured in blood samples. Ovarian tissues were collected and subjected to Hematoxylin-Eosin and Masson's trichrome staining. The expression of angp-2, vegfr-2, smad-2, -4, -6, and tgf-β1 was studied using qRT-PCR analysis. Histopathological examination indicated an increased pattern of atretic follicles in the POI group related to the control rats (P<0.0001).
Results: Data indicated that injection of POI + CD146+ MSCs and CD144+ ECs in POI rats reduced atretic follicles and increased the number of normal follicles (P<0.01). Along with these changes, the content of blue-colored collagen fibers was diminished after cell transplantation. Besides, cell transplantation in POI rats had the potential to reduce increased FSH, and LH levels (P<0.05). In contrast, E2 content was increased in POI + CD146+ MSCs and POI + CD144+ ECs groups compared to control POI rats, indicating restoration of follicular function. CD144+ (smad-2, and -4) and CD146+ (smad-6) cells altered the activity of genes belonging TGF-β signaling pathway. Unlike POI + CD146+ MSCs, aberrant angiogenesis properties were significantly down-regulated in POI + CD144+ ECs related to the control POI group (P<0.05).
Conclusion: The transplantation of bone marrow CD146+ and CD144+ cells can lead to the restoration of ovarian tissue function in POI rats via modulating different mechanisms associated with angiogenesis and fibrosis.
BioimpactsPharmacology, Toxicology and Pharmaceutics-Pharmaceutical Science
CiteScore
4.80
自引率
7.70%
发文量
36
审稿时长
5 weeks
期刊介绍：
BioImpacts (BI) is a peer-reviewed multidisciplinary international journal, covering original research articles, reviews, commentaries, hypotheses, methodologies, and visions/reflections dealing with all aspects of biological and biomedical researches at molecular, cellular, functional and translational dimensions.
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