新型富钙硅酸盐生物活性玻璃陶瓷溶解产物对骨髓基质细胞中VEGF释放的影响

Q1 Materials Science

Biomedical Glasses Pub Date : 2017-12-20 DOI:10.1515/bglass-2017-0010

P. Balasubramanian, R. Detsch, L. Esteban‐Tejeda, A. Grünewald, J. Moya, A. Boccaccini

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引用次数: 9

摘要

摘要本研究评估了一种新型富钙硅酸盐生物活性玻璃的离子溶出产物与市售羟基磷灰石样品（Endobor）相比对体外细胞活性和血管内皮生长因子（VEGF）释放的影响。骨髓基质细胞（ST-2）用不同大小和不同浓度的颗粒上清液（颗粒的0-1wt/vol%）培养48小时。除了体外研究外，细胞形态观察显示所有测试材料释放的产物没有细胞毒性作用。发现来自1wt/vol%浓度的颗粒的上清液增强了ST2细胞的VEGF释放，这在骨愈合过程中作为玻璃的血管形成能力的标志是重要的。

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Influence of dissolution products of a novel Ca-enriched silicate bioactive glass-ceramic on VEGF release from bone marrow stromal cells

Abstract This study evaluated the influence of ionic dissolution products of a novel Ca-enriched silicate bioactive glass compared to commercial available hydroxyapaptite samples (Endobonr) on cell activity and vascular endothelial growth factor (VEGF) release in vitro. Bone marrow stromal cells (ST-2) were cultivated with the supernatant of granules of different sizes and at different concentrations (0-1 wt/vol % of granules) for 48 h. In addition to in vitro studies, Ca-ion release from all as cell morphology observation revealed no cytotoxic effect of the released products from all tested materials. It was found that supernatants from granules in concentrations of 1 wt/vol %enhanced the VEGF release from ST2 cells, which is important as a marker of the vascularisation ability of the glass during the bone healing process.

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来源期刊

Biomedical Glasses Materials Science-Surfaces, Coatings and Films

自引率

0.00%

发文量

审稿时长

17 weeks

期刊介绍： Biomedical Glasses is an international Open Access-only journal covering the field of glasses for biomedical applications. The scope of the journal covers the science and technology of glasses and glass-based materials intended for applications in medicine and dentistry. It includes: Chemistry, physics, structure, design and characterization of biomedical glasses Surface science and interactions of biomedical glasses with aqueous and biological media Modeling structure and reactivity of biomedical glasses and their interfaces Biocompatibility of biomedical glasses Processing of biomedical glasses to achieve specific forms and functionality Biomedical glass coatings and composites In vitro and in vivo evaluation of biomedical glasses Glasses and glass-ceramics in engineered regeneration of tissues and organs Glass-based devices for medical and dental applications Application of glasses and glass-ceramics in healthcare.