主动吸烟供体在储存过程中对白细胞还原的红细胞的红细胞膜耐药性:关注氧化应激

IF 0.2 Q4 MEDICINE, GENERAL & INTERNAL
Dian Eko Astarini, S. A. Jusman, Saptuti Chunaeini
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引用次数: 0

摘要

背景-白细胞减少(去除白细胞)填充红细胞(PRC)通常用于输血,以减少输血后的影响。印度尼西亚的活跃吸烟者数量相对较高;因此,他们中的许多人可能是献血者,因为没有规定将他们排除在外。然而,活跃吸烟者的白细胞减少的PRC风险很高,因为他们可能会受到氧化应激引起的红细胞膜损伤,这阻碍了对抗吸烟产生的自由基。目的:我们的研究考察了氧化应激对活跃吸烟供体在储存过程中产生的白细胞减少的PRC膜抗性的影响。材料和方法:本研究采用描述性和横断面设计。它检查了12名不吸烟(NS)、12名轻度吸烟(LS)和12名中度吸烟(MS)的供体。从供体中产生的Leukoreduced PRC分为三组:NS, LS和ms。我们在储存的第0天至第35天(D0-D35)进行丙二醛(MDA),超氧化物歧化酶(SOD)活性和渗透易碎性试验(OFT)的评估。我们使用非参数统计检验(Kruskal-Wallis和Mann-Whitney)。我们假设p<0.05表示差异有统计学意义。结果- Kruskal-Wallis试验显示三组间MDA、SOD、OFT的D0、D7、D14、D21、D28、D35差异有p < 0.05。这项研究表明,白细胞减少的PRC储存增加了氧化应激。MS组氧化应激最高。结论:红细胞膜脆性和抗氧化性与氧化应激有关。中度吸烟者产生的白细胞诱导的PRC血液成分应禁止反复输注。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Erythrocyte Membrane Resistance Of Actively Smoking Donors In Leukoreduced Packed Red Blood Cells During Storage: Focus On Oxidative Stress
Background — Leukoreduced (with removed white blood cells) packed red blood cells (PRC) are commonly for blood transfusions to reduce post-transfusion effects. The number of active smokers in Indonesia is relatively high; consequently, many of them could be blood donors since no regulations are set to rule them out. However, leukoreduced PRC in active smokers are highly risky as they may undergo erythrocyte membrane damage caused by oxidative stress, which hinders fighting the free radicals generated by smoking. Objective — Our study examined the impact of oxidative stress on membrane resistance of leukoreduced PRC, produced from actively smoking donors, during its storage. Material and Methods − The study was descriptive and cross-sectional in its design. It examined 12 nonsmoking (NS), 12 lightly smoking (LS), and 12 moderately smoking (MS) donors. Leukoreduced PRC produced from donors was separated into three groups: NS, LS, and MS. We performed assessments of malondialdehyde (MDA), superoxide dismutase enzyme (SOD) activity, and osmotic fragility test (OFT) on days 0 through day 35 of storage (D0-D35). We used nonparametric statistical tests (Kruskal-Wallis and Mann-Whitney). We assumed that p<0.05 implied statistically significant difference. Results — The Kruskal-Wallis test demonstrated differences on D0, D7, D14, D21, D28, and D35 between all three groups in MDA, SOD, and OFT, with p < 0.05. This study showed that leukoreduced PRC storage increased oxidative stress. The highest oxidative stress occurred in the MS group. Conclusion — Erythrocyte membrane fragility and resistance correlated with oxidative stress. Blood components of leukoreduced PRC produced from moderate smokers should be banned for repeated transfusions.
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来源期刊
Russian Open Medical Journal
Russian Open Medical Journal MEDICINE, GENERAL & INTERNAL-
CiteScore
0.90
自引率
0.00%
发文量
39
期刊介绍: Russian Open Medical Journal (RusOMJ) (ISSN 2304-3415) is an international peer reviewed open access e-journal. The website is updated quarterly with the RusOMJ’s latest original research, clinical studies, case reports, reviews, news, and comment articles. This Journal devoted to all field of medicine. All the RusOMJ’s articles are published in full on www.romj.org with open access and no limits on word counts. Our mission is to lead the debate on health and to engage, inform, and stimulate doctors, researchers, and other health professionals in ways that will improve outcomes for patients. The RusOMJ team is based mainly in Saratov (Russia), although we also have editors elsewhere in Russian and in other countries.
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