重组基因在毕赤酵母中的表达

James M. Cregg, Ilya Tolstorukov, Anasua Kusari, Anthony Jay Sunga, Knut Madden, Thomas Chappell
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引用次数: 0

摘要

利用从细菌到哺乳动物组织培养细胞的单细胞生物合成特定重组蛋白已成为该行业生物制药产品的主要来源,也是学术研究中各种蛋白质的主要来源。一系列的生物被用于这个目的。其中最新和最有前途的是酵母毕赤酵母。本文详细介绍了利用该酵母表达外源基因和合成重组蛋白的基本方案。特别提供了通过电穿孔将外源载体dna插入酵母的方案,通过翻译后载体扩增(PTVA)方法扩增载体序列,以及在摇瓶培养中生长和表达外源基因。©2018 by John Wiley & Sons, Inc。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Expression of Recombinant Genes in the Yeast Pichia pastoris

The synthesis of specific recombinant proteins using single-celled organisms from bacteria to mammalian tissue culture cells has become a major source of biopharmaceutical products for the industry and a source of a wide variety of proteins for academic research. A range of organisms are utilized for this purpose. One of the newest and most promising of these is the yeast Pichia pastoris. This article provides detailed basic protocols for the expression of heterologous genes and the synthesis of recombinant proteins utilizing this yeast. Specifically provided are protocols for the insertion of foreign vector DNAs into the yeast by electroporation, amplification of vector sequences by the post-translational vector amplification (PTVA) method, and growth and expression of foreign genes in shake flask cultures. © 2018 by John Wiley & Sons, Inc.

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