莪术品种Turina-1乙醇提取物代谢产物谱

Q4 Biochemistry, Genetics and Molecular Biology

Journal of Applied Horticulture Pub Date : 2020-12-25 DOI:10.37855/JAH.2021.V23I01.02

S. Mulyani, B. A. Harsojuwono, I. Satriawan

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引用次数: 1

摘要

通过对姜黄优良品种Turina-1的乙醇提取物进行分析，研究了其代谢产物谱。本研究样品来自印度尼西亚BPPT bogr - indonesia，用乙醇(96%)提取，采用UPLC-QToF-MS/MS系统(Waters)，质谱:XEVO-G2QTOF (Waters)， ESI正分辨模式，梯度法，流动相:水、甲酸、乙腈。研究发现13种代谢产物分别为:去甲氧基姜黄素-2(48.23%)、α-姜黄酮(19.623%)、姜黄素(18.550%)、双去甲氧基姜黄素-3(9.064%)、姜黄素-1(1.706%)和其他含量小于1%的化合物(山奈酚3- o -葡萄糖基鼠李糖半乳糖苷、去甲氧基姜黄素、ar-姜黄酮、双去甲氧基姜黄素、a-松油烯、l-酪氨酸、l-丙氨酸和l-丝氨酸)。基于本研究，Turina-1乙醇提取物中具有抗氧化剂潜力的主要代谢物是姜黄素。

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Metabolite profile of ethanol extract of Curcuma domestica Val. variety Turina-1

The metabolite profile of Curcuma domestica Val. variety Turina-1, one of the superior varieties of turmeric was studied by analysing ethanol extract of the variety. The samples for this research were obtained from BPPT Bogor-Indonesia and were extracted using ethanol (96 %) and then analyzed using UPLC-QToF-MS/MS System (Waters), mass spectrometry: XEVO-G2QTOF (Waters), in ESI positive resolution mode, using gradient method with mobile phase: water, formic acid and acetonitrile. The study revealed 13 metabolites viz., Demethoxycurcumin-2 (48.23 %), α-Turmerone (19.623 %), Curcumin (18.550 %), Bisdemethoxycurcumin-3 (9.064 %), Curcumin-1, (1.706 %), and other compounds with amount less than 1 % (Kaempferol 3-O-glucosyl-rhamnosyl-galactoside, Demethoxycurcumin, ar-Turmerone, Bisdemethoxycurcumin, a-Terpinolene, L-Tyrosine, L-Alanine and L-serine). Based on this research, the main metabolite in the ethanol extract of Turina-1 having the potential as antioxidants is the curcuminoids.

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来源期刊

Journal of Applied Horticulture Agricultural and Biological Sciences-Horticulture

CiteScore

0.90

自引率

0.00%

发文量

期刊介绍： The Journal of Applied Horticulture (JAH) is an official publication of the Society for the Advancement of Horticulture, founded in 1999. JAH is a triannual publication, publishes papers of original work (or results), & rapid communications and reviews on all aspects of Horticultural Science which can contribute to fundamental and applied research on horticultural plants and their related products. The essential contents of manuscripts must not have been published in other refereed publications. Submission of a manuscript to the Journal implies no concurrent submission elsewhere.