溶酶体膜蛋白LAMP‐2对于PINK1/Parkin介导的有丝分裂是必不可少的

IF 3 4区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

FEBS Letters Pub Date : 2019-11-06 DOI:10.1002/1873-3468.13663

Xuemei Liu, Xinyi Liao, Xiyun Rao, Bin Wang, Jun Zhang, Ge Xu, Xuejun Jiang, Xia Qin, Chengzhi Chen, Zhen Zou

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引用次数: 5

摘要

选择性自噬消除异常线粒体，称为线粒体自噬，可由激酶PINK1和泛素连接酶Parkin调节。溶酶体相关膜蛋白2 (LAMP‐2)在非选择性自噬、伴侣介导的自噬和RNA/DNA降解的选择性自噬中发挥着多种功能。在本研究中，我们研究了LAMP‐2是否在PINK1/Parkin介导的有丝分裂中发挥重要作用。结果清楚地表明，在稳定表达Parkin的HeLa细胞中，敲低LAMP‐2并不会导致线粒体自噬缺陷，这表明在PINK1/Parkin介导的线粒体自噬中，LAMP‐2是必不可少的。本研究首次确定了LAMP‐2在PINK1/Parkin介导的线粒体自噬中的潜在作用，从而为线粒体自噬的复杂过程提供了更多的见解。

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The lysosomal membrane protein LAMP‐2 is dispensable for PINK1/Parkin‐mediated mitophagy

Selective autophagy for the elimination of aberrant mitochondria, termed mitophagy, can be regulated by the kinase PINK1 and the ubiquitin ligase Parkin. The lysosome‐associated membrane protein 2 (LAMP‐2) plays diverse functions in non‐selective autophagy, chaperone‐mediated autophagy and selective autophagy for the degradation of RNA/DNA. In the present study, we investigated whether LAMP‐2 plays important roles during PINK1/Parkin‐mediated mitophagy. The results obtained clearly show that knockdown of LAMP‐2 does not cause defects in mitophagy in HeLa cells stably expressing Parkin, indicating that LAMP‐2 is dispensable for PINK1/Parkin‐mediated mitophagy. The present study is the first to determine the potential role of LAMP‐2 in PINK1/Parkin‐mediated mitophagy, thereby providing more insight into the sophisticated process of mitophagy.

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来源期刊

FEBS Letters 生物-生化与分子生物学

CiteScore

6.60

自引率

2.90%

发文量

303

审稿时长

1 months

期刊介绍： FEBS Letters is one of the world''s leading journals in molecular biology and is renowned both for its quality of content and speed of production. Bringing together the most important developments in the molecular biosciences, FEBS Letters provides an international forum for Minireviews, Research Letters and Hypotheses that merit urgent publication.