肝细胞生长因子基因转染间充质干细胞对二甲基亚硝胺诱导大鼠肝纤维化的影响

IF 1.8 4区生物学 Q4 CELL BIOLOGY

Growth factors Pub Date : 2019-07-04 DOI:10.1080/08977194.2019.1652399

S. Moon, C. Lee, See-Hyoung Park, Myeong Jin Nam

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引用次数: 18

摘要

目前，人间充质干细胞(MSCs)移植已成为一种潜在的肝硬化细胞治疗方法。肝细胞生长因子(HGF)在肝脏再生中起着重要作用。本研究旨在探讨hgf转染人脐带血源性间充质干细胞对二甲亚硝胺(DMN)诱导大鼠肝纤维化的治疗作用。将转染hgf的间充质干细胞移植到dmn诱导的肝纤维化大鼠体内。hgf转染的MSCs中h2o2诱导的细胞毒性、细胞凋亡和细胞内活性氧减少。促凋亡蛋白，如cleaved poly (adp -核糖)聚合酶和cleaved caspase-3，在hgf转染的MSCs中减少。生化分析显示，hgf转染的间充质干细胞移植后，大鼠肝纤维化组织中谷草转氨酶和丙氨酸转氨酶水平降低。三色染色显示hgf转染的MSCs减轻了肝损伤。综上所述，我们的研究表明hgf转染的MSCs对dmn诱导的大鼠肝纤维化具有治疗作用。

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Effects of hepatocyte growth factor gene-transfected mesenchymal stem cells on dimethylnitrosamine-induced liver fibrosis in rats

Abstract Nowadays, transplantation of human mesenchymal stem cells (MSCs) has emerged as a potential cellular therapy for liver cirrhosis. Hepatocyte growth factor (HGF) plays an important role in the regeneration of the liver. The objective of the study was to investigate the therapeutic effect of HGF-transfected human umbilical cord blood-derived MSCs on dimethylnitrosamine (DMN)-induced liver fibrosis in rats. HGF-transfected MSCs were transplanted into rats with DMN-induced liver fibrosis. H2O2-induced cytotoxicity, apoptosis and intracellular reactive oxygen species were reduced in HGF-transfected MSCs in HGF-transfected MSCs. Pro-apoptotic proteins, such as cleaved poly (ADP-ribose) polymerase and cleaved caspase-3, were decreased in HGF-transfected MSCs. Biochemical analysis showed that the levels of aspartate aminotransferase and alanine aminotransferase were decreased after transplantation of HGF-transfected MSCs in rat fibrosis. Trichrome staining showed that HGF-transfected MSCs reduced liver damage. Taken together, our study indicated that HGF-transfected MSCs have therapeutic effects on DMN-induced liver fibrosis in rats.

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来源期刊

Growth factors 生物-内分泌学与代谢

CiteScore

2.60

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Growth Factors is an international and interdisciplinary vehicle publishing new knowledge and findings on the regulators of cell proliferation, differentiation and survival. The Journal will publish research papers, short communications and reviews on current developments in cell biology, biochemistry, physiology or pharmacology of growth factors, cytokines or hormones which improve our understanding of biology or medicine. Among the various fields of study topics of particular interest include: •Stem cell biology •Growth factor physiology •Structure-activity relationships •Drug development studies •Clinical applications