用葡萄糖条和血糖仪快速测定辣根(Armoracia rusticana)中总硫代葡萄糖苷含量的方法的开发和验证。

IF 1.2 4区生物学 Q2 Agricultural and Biological Sciences

Journal of Applied Botany and Food Quality-Angewandte Botanik Pub Date : 2018-10-17 DOI:10.5073/JABFQ.2018.091.031

N. Meitinger, W. Kreis

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引用次数: 1

摘要

介绍了一种在不到10分钟内快速测定新鲜辣根中硫代葡萄糖苷总含量的方法。该方法包括以下步骤：1。用4%磷酸处理辣根以避免酶促降解内源性硫代葡萄糖苷，2。提取物的中和和使用商业血糖仪测定游离葡萄糖，3。外源芥子糖苷酶水解硫代葡萄糖苷的研究。再次使用血糖仪检测释放的葡萄糖，以及5。基于所确定的两个葡萄糖值之间的差异来计算硫代葡萄糖苷含量。将新开发的测定法（“TC快速试验”）与硫代葡萄糖苷分析的标准高效液相色谱法进行了比较。

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Development and validation of a quick assay for the total glucosinolate content in horseradish (Armoracia rusticana) using glucose strips and a blood glucose meter.

A quick assay to determine the total glucosinolate content of fresh horseradish roots in less than 10 minutes is described. The method involves the following steps: 1. Maceration of horseradish root with 4% phosphoric acid to avoid enzymatic degradation of endogenous glucosinolates, 2. neutralization of the extract and determination of free glucose using a commercial blood glucose meter, 3. enzymatic hydrolysis of the glucosinolates by exogenous myrosinase, 4. detection of released glucose, again using a blood glucose meter, and 5. calculation of the glucosinolate content on the basis of the difference between the two glucose values determined. The newly developed assay (‘ITC quick test’) was compared with a standard high-performance liquid chromatographic method for glucosinolate analysis.

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来源期刊

Journal of Applied Botany and Food Quality-Angewandte Botanik 生物-植物科学

CiteScore

2.50

自引率

0.00%

发文量

审稿时长

20 weeks

期刊介绍： The Journal of Applied Botany and Food Quality is the Open Access journal of the German Society for Quality Research on Plant Foods and the Section Applied Botany of the German Botanical Society. It provides a platform for scientists to disseminate recent results of applied plant research in plant physiology and plant ecology, plant biotechnology, plant breeding and cultivation, phytomedicine, plant nutrition, plant stress and resistance, plant microbiology, plant analysis (including -omics techniques), and plant food chemistry. The articles have a clear focus on botanical and plant quality aspects and contain new and innovative information based on state-of-the-art methodologies.