转基因BT玉米防治棉铃虫的效果研究

Fernando Belezini Vinha, C. Zambiasi, Murilo Gaspar Litholdo, Abílio José Meiato Barrionuevo Oliveira, A. Pinto
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引用次数: 0

摘要

2013年在巴西发现的毛毛虫棉铃虫(鳞翅目:夜蛾科),由于其破坏力,农民非常担心。诱导昆虫抗性的转基因植物技术是控制毛毛虫的重要盟友。本研究比较了转Bt基因玉米对棉铃虫不同幼虫龄期的控制效果。检测了以下基因型:(1)非Bt玉米等位杂交种(对照);(2) Cry1F;(3) Cry1F+Cry1A.105+Cry2Ab2;(4) Cry1A.105+Cry2Ab2;(5) Cry1Ab+Cry1F;(6) Cry1Ab+Vip3Aa20。实验设计以随机分组的方式组织,共有8个重复,每个重复由一个装有5株人工感染2龄、3龄或4龄幼虫的植物的花盆组成。在侵扰后1、3、7、10和14天,对植物进行落叶评估。在3龄幼虫的生物测定中,鸟类的捕食率很高,但直到感染后7天,所有转基因玉米都对害虫有效。所有转基因Bt玉米均能有效控制棉铃虫幼虫的龄期。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
EFFECTIVENESS OF TRANSGENIC BT MAIZE TO CONTROL Helicoverpa armigera (LEPIDOPTERA: NOCTUIDAE)
The caterpillar Helicoverpa armigera (Hubner) (Lepidoptera: Noctuidae), identified in Brazil in 2013, greatly worries farmers because of its destructive power. The technology of transgenic plants that induces resistance to insects is a great ally for controlling caterpillars. In this study we compared the performance of transgenic Bt maize in the controlling of different larval instars of H. armigera in semi-field seedlings. There were tested the following genotypes: (1) Non-Bt maize Iso-hybrid (Control); (2) Cry1F; (3) Cry1F+ Cry1A.105 + Cry2Ab2; (4) Cry1A.105 + Cry2Ab2; (5) Cry1Ab + Cry1F; (6) Cry1Ab + Vip3Aa20. The experimental design was organized in randomized blocks with eight replications, each consisting of a pot with 5 plants artificially infested with larvae of 2nd, 3rd or 4th instar. At 1, 3, 7, 10 and 14 days after infestation, plants were evaluated for defoliation. In the 3rd instar larvae bioassay, there was high predation by birds, but until seven days after infestation all transgenic maize were efficient to the pest. All transgenic Bt maize were effective in the control of tested H. armigera larvae instars.
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