一种改善哺乳动物胚胎体外发育的替代方法：培养无Pellucida带胚胎。

IF 1.2 4区医学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Cellular reprogramming Pub Date : 2022-05-03 DOI:10.1089/cell.2022.0012

Sarah Madani, Z. Machaty, G. Vajta

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引用次数: 3

摘要

越来越多的数据证明，透明带的存在对哺乳动物胚胎的产生，包括成熟、受精和胚胎培养并不是必需的。事实上，体外产生的胚胎的透明带结构与体内的透明带有很大的不同，这影响了代谢，并且在孵化时需要付出不成比例的努力才能打开。本文旨在关注这一领域，促进无带胚培养的研究。在家畜中，专门设计的无带胚胎培养系统的广泛应用证明了该方法的可行性。它可能为转基因研究和人类辅助生殖开辟新的可能性和提高效率。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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An Alternative Way to Improve Mammalian Embryo Development In Vitro: Culture of Zona Pellucida-Free Embryos.

An increasing number of data proves that the presence of the zona pellucida is not essential to mammalian embryo production, including maturation, fertilization, and embryo culture. In fact, the structure of the zona pellucida of in vitro-produced embryos differs significantly from its in vivo counterpart, influencing metabolism and requiring disproportionate efforts to crack open at the time of hatching. This review aims to focus attention on this field and stimulate research in zona-free embryo culture. In domestic animals, extensive application of purpose-designed culture systems for zona-free embryos proved the feasibility of this approach. It may open new possibilities and increase efficiency in both transgenic research and human-assisted reproduction.

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来源期刊

Cellular reprogramming CELL & TISSUE ENGINEERING-BIOTECHNOLOGY & APPLIED MICROBIOLOGY

CiteScore

2.50

自引率

6.20%

发文量

审稿时长

3 months

期刊介绍： Cellular Reprogramming is the premier journal dedicated to providing new insights on the etiology, development, and potential treatment of various diseases through reprogramming cellular mechanisms. The Journal delivers information on cutting-edge techniques and the latest high-quality research and discoveries that are transforming biomedical research. Cellular Reprogramming coverage includes: Somatic cell nuclear transfer and reprogramming in early embryos Embryonic stem cells Nuclear transfer stem cells (stem cells derived from nuclear transfer embryos) Generation of induced pluripotent stem (iPS) cells and/or potential for cell-based therapies Epigenetics Adult stem cells and pluripotency.