Muhammed Bekmezci, Ramazan Bayat, Merve Akin, Zeynep Kazel Coguplugil, Fatih Sen
{"title":"用于检测胰腺癌生物标志物miR-141的可兼容手机的改进丝网印刷电化学生物传感器设计","authors":"Muhammed Bekmezci, Ramazan Bayat, Merve Akin, Zeynep Kazel Coguplugil, Fatih Sen","doi":"10.1007/s42823-023-00545-9","DOIUrl":null,"url":null,"abstract":"<div><p>MicroRNAs (miRNAs) are emerging materials as ideal biomarkers for noninvasive cancer detection in the early phase. In this article, a simple and label-free electrochemical miRNA biosensor was developed. A single-stranded DNA (ss-DNA) probes were successfully mapped to f-MWCNT and hybridized with the target miR-141 sequence. The optimum peak points of the obtained hybridization were determined using Cyclic Voltammetry (CV) and Differential Pulse Voltammetry (DPV) methods. Significant peaks were observed in the results, depending on miR-141 at different concentrations. The linear relationship (<i>ν</i>) between redox peak currents (Ip) and scanning rate indicated that electron transfer (ET) between miR-141 and the electrode surface was accomplished successfully. In DPV measurements, miR-141 was measured with a low detection limit (LOD) in the 1.3–12 nM concentration range, and the LOD and limit of quantification (LOQ) results were found to be 3 and 9.1 pM, respectively. Besides, selectivity test was investigated for the biosensor using different target analytes and a significant difference in value was observed between the peak currents of miR-141, and other target molecules. This developed strategy has been found to detect miR-141 sensitively, selectively and without tags, and its integration into mobile devices has been successfully carried out.</p></div>","PeriodicalId":506,"journal":{"name":"Carbon Letters","volume":"33 6","pages":"1863 - 1873"},"PeriodicalIF":5.5000,"publicationDate":"2023-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"2","resultStr":"{\"title\":\"Modified screen-printed electrochemical biosensor design compatible with mobile phones for detection of miR-141 used to pancreatic cancer biomarker\",\"authors\":\"Muhammed Bekmezci, Ramazan Bayat, Merve Akin, Zeynep Kazel Coguplugil, Fatih Sen\",\"doi\":\"10.1007/s42823-023-00545-9\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>MicroRNAs (miRNAs) are emerging materials as ideal biomarkers for noninvasive cancer detection in the early phase. In this article, a simple and label-free electrochemical miRNA biosensor was developed. A single-stranded DNA (ss-DNA) probes were successfully mapped to f-MWCNT and hybridized with the target miR-141 sequence. The optimum peak points of the obtained hybridization were determined using Cyclic Voltammetry (CV) and Differential Pulse Voltammetry (DPV) methods. Significant peaks were observed in the results, depending on miR-141 at different concentrations. The linear relationship (<i>ν</i>) between redox peak currents (Ip) and scanning rate indicated that electron transfer (ET) between miR-141 and the electrode surface was accomplished successfully. In DPV measurements, miR-141 was measured with a low detection limit (LOD) in the 1.3–12 nM concentration range, and the LOD and limit of quantification (LOQ) results were found to be 3 and 9.1 pM, respectively. Besides, selectivity test was investigated for the biosensor using different target analytes and a significant difference in value was observed between the peak currents of miR-141, and other target molecules. This developed strategy has been found to detect miR-141 sensitively, selectively and without tags, and its integration into mobile devices has been successfully carried out.</p></div>\",\"PeriodicalId\":506,\"journal\":{\"name\":\"Carbon Letters\",\"volume\":\"33 6\",\"pages\":\"1863 - 1873\"},\"PeriodicalIF\":5.5000,\"publicationDate\":\"2023-07-06\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"2\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Carbon Letters\",\"FirstCategoryId\":\"88\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s42823-023-00545-9\",\"RegionNum\":3,\"RegionCategory\":\"材料科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"CHEMISTRY, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Carbon Letters","FirstCategoryId":"88","ListUrlMain":"https://link.springer.com/article/10.1007/s42823-023-00545-9","RegionNum":3,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
Modified screen-printed electrochemical biosensor design compatible with mobile phones for detection of miR-141 used to pancreatic cancer biomarker
MicroRNAs (miRNAs) are emerging materials as ideal biomarkers for noninvasive cancer detection in the early phase. In this article, a simple and label-free electrochemical miRNA biosensor was developed. A single-stranded DNA (ss-DNA) probes were successfully mapped to f-MWCNT and hybridized with the target miR-141 sequence. The optimum peak points of the obtained hybridization were determined using Cyclic Voltammetry (CV) and Differential Pulse Voltammetry (DPV) methods. Significant peaks were observed in the results, depending on miR-141 at different concentrations. The linear relationship (ν) between redox peak currents (Ip) and scanning rate indicated that electron transfer (ET) between miR-141 and the electrode surface was accomplished successfully. In DPV measurements, miR-141 was measured with a low detection limit (LOD) in the 1.3–12 nM concentration range, and the LOD and limit of quantification (LOQ) results were found to be 3 and 9.1 pM, respectively. Besides, selectivity test was investigated for the biosensor using different target analytes and a significant difference in value was observed between the peak currents of miR-141, and other target molecules. This developed strategy has been found to detect miR-141 sensitively, selectively and without tags, and its integration into mobile devices has been successfully carried out.
期刊介绍：
Carbon Letters aims to be a comprehensive journal with complete coverage of carbon materials and carbon-rich molecules. These materials range from, but are not limited to, diamond and graphite through chars, semicokes, mesophase substances, carbon fibers, carbon nanotubes, graphenes, carbon blacks, activated carbons, pyrolytic carbons, glass-like carbons, etc. Papers on the secondary production of new carbon and composite materials from the above mentioned various carbons are within the scope of the journal. Papers on organic substances, including coals, will be considered only if the research has close relation to the resulting carbon materials. Carbon Letters also seeks to keep abreast of new developments in their specialist fields and to unite in finding alternative energy solutions to current issues such as the greenhouse effect and the depletion of the ozone layer. The renewable energy basics, energy storage and conversion, solar energy, wind energy, water energy, nuclear energy, biomass energy, hydrogen production technology, and other clean energy technologies are also within the scope of the journal. Carbon Letters invites original reports of fundamental research in all branches of the theory and practice of carbon science and technology.
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