鉴定和鉴定导致巴基斯坦稻米变色的主要致病病原体

IF 0.7 4区 农林科学 Q3 AGRICULTURE, MULTIDISCIPLINARY
M. Ashfaq
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引用次数: 1

摘要

粮食变色影响着全球水稻作物的品质、数量和产量。在此,形态学,分子和致病的病原特征引起稻米变色。对20个水稻基因型进行田间侵染鉴定,用12对非连锁多态性SSR引物扫描进行遗传多样性分析。从变色水稻穗中分离病原菌,对其进行DNA测序研究。采用通用引物对对分离的病原菌进行DNA测序。此外,利用科赫的假设在形态学上重新确认了新的病原体。地衣芽孢杆菌是引起水稻籽粒变色病的主要病原菌。发现的抗水稻变色基因型包括Super-basmati、Rondo、Gulfmont、KSK-133和L-203,可作为选育抗水稻变色品种的潜在遗传资源。本研究为研究人员和农民利用地衣芽孢杆菌防治水稻病害提供了参考。本研究对科研人员和科学界利用本研究开展新的研究项目同样有益
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Identifying and characterizing the main causal pathogen responsible for rice grain discoloration in Pakistan
Grain discoloration affects grain quality, quantity, and yield of rice crop worldwide. Herein, morphological, molecular, and pathogenic characterizations of the pathogen causing rice grain discoloration were performed. A set of 20 rice genotypes were scored for disease infestation in field and scanned with 12 unlinked polymorphic SSR primer pairs for genetic diversity assay. Pathogens from the discolored rice panicles were isolated to investigate the disease-causing pathogen on DNA sequencing basis. The universal primer pairs were used for DNA sequencing of the isolated pathogen. Furthermore, the new pathogen was reconfirmed morphologically by using Koch’s postulates. The bacterial species Bacillus licheniformis was identified as a major pathogen causing grain discoloration disease in rice. The genotypes found to be resistant to rice grain discoloration include Super-basmati, Rondo, Gulfmont, KSK-133 and L-203 which can be used as potential genetic resources for breeding rice grain discoloration resistant cultivars. The study provided information to the researchers and farmers for managing and controlling rice grain disease by the adoption of Bacillus licheniformis. The study is equally beneficial both for researchers and scientific community to start up a new research program by utilization of this study
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来源期刊
Pakistan Journal of Agricultural Sciences
Pakistan Journal of Agricultural Sciences AGRICULTURE, MULTIDISCIPLINARY-
CiteScore
1.80
自引率
25.00%
发文量
18
审稿时长
6-12 weeks
期刊介绍: Pakistan Journal of Agricultural Sciences is published in English four times a year. The journal publishes original articles on all aspects of agriculture and allied fields.
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