车前草皂苷通过抑制PI3K/AKT和ERK通路减少胃癌细胞的迁移和侵袭

IF 0.4 4区医学 Q4 NUTRITION & DIETETICS

Current Topics in Nutraceutical Research Pub Date : 2023-04-11 DOI:10.37290/ctnr2641-452x.21:175-181

Cheng-fang Tang, Yuanyuan Wang, Jing Huang, Xiaohong Lv

{"title":"车前草皂苷通过抑制PI3K/AKT和ERK通路减少胃癌细胞的迁移和侵袭","authors":"Cheng-fang Tang, Yuanyuan Wang, Jing Huang, Xiaohong Lv","doi":"10.37290/ctnr2641-452x.21:175-181","DOIUrl":null,"url":null,"abstract":"Gastric cancer is a common malignancy with a poor survival rate. Plantamajoside, a phenylethanoid glycoside from Plantago asiatica, has been shown to inhibit the progression of many different types of cancer; however, its effect on gastric cancer remains to be explored. To this end, the effect of plantamajoside on the proliferation, apoptosis, stemness, mobility, and invasion of gastric cancer was evaluated by cell survival assay, flow cytometry, colony formation, sphere formation, transwell, and Western blot assays. Also, the potential molecular mechanism was addressed by Western blot assays for the expression of various signal proteins. Plantamajoside reduced cell viability, enhanced apoptosis, reduced cell mobility, and increased invasion of both AGS and HS746T cells in a dose-dependent manner. Plantamajoside also decreased the diameter of the spheroid and the relative expression of octamer-binding transcription factor-4 and the cluster of differentiation 44 proteins in both AGS and HS746T cells. Furthermore, plantamajoside downregulated the relative protein levels of p-PI3K/ PI3K, p-AKT/AKT, and p-ERK/ERK in both AGS and HS746T cells.","PeriodicalId":10976,"journal":{"name":"Current Topics in Nutraceutical Research","volume":null,"pages":null},"PeriodicalIF":0.4000,"publicationDate":"2023-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Plantamajoside Reduces Gastric Cancer Cell Migration and Invasion by Inhibiting PI3K/AKT and ERK Pathways\",\"authors\":\"Cheng-fang Tang, Yuanyuan Wang, Jing Huang, Xiaohong Lv\",\"doi\":\"10.37290/ctnr2641-452x.21:175-181\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Gastric cancer is a common malignancy with a poor survival rate. Plantamajoside, a phenylethanoid glycoside from Plantago asiatica, has been shown to inhibit the progression of many different types of cancer; however, its effect on gastric cancer remains to be explored. To this end, the effect of plantamajoside on the proliferation, apoptosis, stemness, mobility, and invasion of gastric cancer was evaluated by cell survival assay, flow cytometry, colony formation, sphere formation, transwell, and Western blot assays. Also, the potential molecular mechanism was addressed by Western blot assays for the expression of various signal proteins. Plantamajoside reduced cell viability, enhanced apoptosis, reduced cell mobility, and increased invasion of both AGS and HS746T cells in a dose-dependent manner. Plantamajoside also decreased the diameter of the spheroid and the relative expression of octamer-binding transcription factor-4 and the cluster of differentiation 44 proteins in both AGS and HS746T cells. Furthermore, plantamajoside downregulated the relative protein levels of p-PI3K/ PI3K, p-AKT/AKT, and p-ERK/ERK in both AGS and HS746T cells.\",\"PeriodicalId\":10976,\"journal\":{\"name\":\"Current Topics in Nutraceutical Research\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.4000,\"publicationDate\":\"2023-04-11\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Current Topics in Nutraceutical Research\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.37290/ctnr2641-452x.21:175-181\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"NUTRITION & DIETETICS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current Topics in Nutraceutical Research","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.37290/ctnr2641-452x.21:175-181","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"NUTRITION & DIETETICS","Score":null,"Total":0}

引用次数: 0

摘要

胃癌是一种常见的恶性肿瘤，生存率很低。车前草皂苷是一种来自车前草的苯乙醇苷，已被证明可以抑制许多不同类型癌症的进展;然而，其对胃癌的影响仍有待探讨。为此，采用细胞存活试验、流式细胞术、集落形成、球形成、transwell、Western blot等方法，评价了金跖皂苷对胃癌细胞增殖、凋亡、干性、移动性和侵袭性的影响。此外，通过Western blot检测各种信号蛋白的表达，探讨了潜在的分子机制。车前草皂苷降低细胞活力，增强细胞凋亡，降低细胞流动性，增加AGS和HS746T细胞的侵袭，并呈剂量依赖性。车前草皂苷还能降低AGS和HS746T细胞的球体直径、八聚体结合转录因子-4和分化44蛋白簇的相对表达量。此外，车前草皂苷下调AGS和HS746T细胞中p-PI3K/ PI3K、p-AKT/AKT和p-ERK/ERK的相对蛋白水平。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Plantamajoside Reduces Gastric Cancer Cell Migration and Invasion by Inhibiting PI3K/AKT and ERK Pathways

Gastric cancer is a common malignancy with a poor survival rate. Plantamajoside, a phenylethanoid glycoside from Plantago asiatica, has been shown to inhibit the progression of many different types of cancer; however, its effect on gastric cancer remains to be explored. To this end, the effect of plantamajoside on the proliferation, apoptosis, stemness, mobility, and invasion of gastric cancer was evaluated by cell survival assay, flow cytometry, colony formation, sphere formation, transwell, and Western blot assays. Also, the potential molecular mechanism was addressed by Western blot assays for the expression of various signal proteins. Plantamajoside reduced cell viability, enhanced apoptosis, reduced cell mobility, and increased invasion of both AGS and HS746T cells in a dose-dependent manner. Plantamajoside also decreased the diameter of the spheroid and the relative expression of octamer-binding transcription factor-4 and the cluster of differentiation 44 proteins in both AGS and HS746T cells. Furthermore, plantamajoside downregulated the relative protein levels of p-PI3K/ PI3K, p-AKT/AKT, and p-ERK/ERK in both AGS and HS746T cells.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Current Topics in Nutraceutical Research 医学-药学

CiteScore

1.10

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Current Topics in Nutraceutical Research is an international, interdisciplinary broad-based peer reviewed scientific journal for critical evaluation of research on chemistry, biology and therapeutic applications of nutraceuticals and functional foods. The major goal of this journal is to provide peer reviewed unbiased scientific data to the decision makers in the nutraceutical and food industry to help make informed choices about development of new products. To this end, the journal will publish two types of review articles. First, a review of preclinical research data coming largely from animal, cell culture and other experimental models. Such data will provide basis for future product development and/or human research initiatives. Second, a critical evaluation of current human experimental data to help market and deliver the product for medically proven use. This journal will also serve as a forum for nutritionists, internists, neurologists, psychiatrists, and all those interested in preventive medicine. The common denominator of all of the topic to be covered by the journal must include nutraceuticals and/functional food. The following is an example of some specific areas that may be of interest to the journal. i) Role of vitamins, minerals, antioxidants and phytonutrients on cardiovascular health, cancer, diabetes, ocular health, mental health, men’s health, women’s health, infant nutrition, ii) Role of herbals on human health, iii) Dietary supplements and sleep, iv) Components of diet that may have beneficial effect on human health, v) regulation of apoptosis and cell viability, vi) Isolation and characterization of bioactive components from functional foods, vii) Nutritional genomics, and viii) Nutritional proteomics.