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引用次数: 3
摘要
为了在毛细管电泳(CE)和微芯片电泳(MCE)中实现高灵敏度的磷酸肽分析，采用电渗透泵(LVSEP)在聚乙烯吡罗烷酮(PVP)动态包被的毛细血管中进行大容量样品堆积，以抑制电渗透流动。在对牛β -酪蛋白单磷酸肽(MPP)的分析中，在20 mM HEPES缓冲液(pH 9.0)中使用0.2% PVP，在CE和MCE中分别以260和400的敏感增强因子(SEF)获得了较好的富集效果。
LVSEP Analysis of Phosphopeptides in Dynamically PVP-Coated Capillaries and Microchannels
To achieve highly sensitive analyses of phosphopeptides with simple experimental procedures in capillary electrophoresis (CE) and microchip electrophoresis (MCE), large-volume sample stacking with an electroosmotic flow pump (LVSEP) was performed in dynamically poly(vinyl pyrrolidone) (PVP)-coated capillaries to suppress the electroosmotic flow. In the analysis of monophosphopeptide (MPP) from bovine β -casein, good enrichments were attained with the sensitive enhancement factor (SEF) of 260 and 400 in CE and MCE, respectively, by using 0.2% PVP in 20 mM HEPES buffer (pH 9.0) in LVSEP.
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