P. Desiyamani, M. S. Ali, V. Anuradha, N. Yogananth, J. Chitra
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引用次数: 0
摘要
测定马齿苋吊脚根和马齿苋叶甲醇提取液对登革热幼虫的杀幼虫活性。用二甲基亚砜(DMSO)溶解短茎根和马齿苋叶的甲醇提取物,制备分级系列浓度。将25批登革热4龄早期幼虫转移到250毫升搪瓷碗中,碗中装有199毫升蒸馏水和1毫升植物提取物(0.01-0.1毫克)。每个实验分三个重复进行。对照组仅由1 mL DMSO和99 mL蒸馏水组成。24小时后,死亡率用以下公式确定:提取物的百分比=提取物的重量/植物材料的重量X100。分级叶提取物显示出最大的杀幼虫活性分级提取物3显示出最小的LC50值水平(0.051±0.2(0.41-0.602),马齿苋吊脚根和马齿苋叶对登革热幼虫的甲醇分级提取物具有开发杀幼虫活性的活性化合物。
Mosquito Larvicidal Activity of Fractionated Methanolic Extractof Rhizophora mucronata Stilt Root and Sesuvium portulacastrum against Dengue Larvae
To determine the larvicidal activity of fractionated methanolic extract of stilt root of Rhizophora mucronata and leaf of Sessuvium portulacastrum against dengue larvae. Fractionated methanolic extracts of stilt root of Rhizophora mucronata and leaf of Sessuvium portulacastrum were dissolved in dimethylsulfoxide (DMSO) to prepare a graded series of concentration. Batches of 25 early 4 th instars larvae of dengue were transferred to 250 mL enamel bowl containing 199 mL of distilled water and 1 mL of plant extracts (0.01-0.1 mg). Each experiment was conducted in three replicates. A control group consisted of 1 mL of DMSO and 99 mL of distilled water only. After 24 h, the percentage of mortality was identified with the formula: % of extraction= wt of the extract/wt of the plant material X 100. The fractionated leaf extracts showed maximum larvicidal activity fractionated extract 3 showed minimum level of LC 50 value (0.051±0.2 (0.41-0.602). Simultaneously, the fractionated stilt root extract of Rhizophora mucronata showed maximum larvicidal activity and (RF-2) showed minimum level of LC 50 value (0.51±0.29 (0.45-0.65). It can be concluded from the present study that, the fractionated methanolic extracts of stilt root of Rhizophora mucronata and leaf of Sessuvium portulacastrum against dengue larvae possess active compounds for development of larvicidal activity.