接骨木提取物对顺铂诱导的人肝癌（HepG2）细胞毒性的抗氧化作用

Q4 Pharmacology, Toxicology and Pharmaceutics

Iranian Journal of Toxicology Pub Date : 2021-04-01 DOI:10.32598/IJT.15.2.764.1

Amin Sharifan, M. Etebari, B. Zolfaghari, M. Aliomrani

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引用次数: 0

摘要

方法：采用浸渍法提取接骨松树皮乙醇提取物（BHAEPE）。用Folin-ciocalteu试剂测定了BHAEPE的总酚含量。采用Clevenger加氢蒸馏法获得了接骨松针叶挥发油（NVOPE）。采用气相色谱-质谱联用技术对NVOPE的主要成分进行了鉴定。我们使用DPPH测定法来研究NVOPE和BHAEPE的抗氧化活性。同时，MTT法检测BHAEPE和NVOPE对顺铂的保护作用。

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Antioxidant Effects of Pinus eldarica Extracts Against Cisplatin-induced Cytotoxicity in Human Hepatoma (HepG2) Cell Line

Methods: Using the maceration technique, we obtained the ethanolic extract of Pinus eldarica’s bark (BHAEPE). Folin-ciocalteu reagent was used to determine the total phenolic content of BHAEPE. The Needle’s Volatile Oil of Pinus eldarica (NVOPE) was obtained by Clevenger hydrodistillation method. The main components of NVOPE were identified by Gas Chromatography and Mass Spectrophotometry (GC-MS). We used DPPH assay to investigate the antioxidant activity of both NVOPE and BHAEPE. Also, MTT assay was performed to test the protective effects of both BHAEPE and NVOPE against cisplatin.

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来源期刊

Iranian Journal of Toxicology Environmental Science-Health, Toxicology and Mutagenesis

CiteScore

1.60

自引率

0.00%

发文量

审稿时长

9 weeks