{"title":"藏红花素通过调节Nrf2转录活性抑制rmTBI小鼠模型炎症诱导的细胞凋亡","authors":"Marwa Salem, Mariam Shaheen, Jamilah Borjac","doi":"10.1016/j.phanu.2022.100308","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><p><span><span>Repetitive mild traumatic brain injury (rmTBI) has been considered a serious health issue. </span>Crocin<span>, a bioactive carotenoid in </span></span><span><em>Crocus sativus</em></span><span> (saffron) is well known for its anti-inflammatory and anti-oxidant properties. The aim of this study is to investigate the neuroprotective role of crocin in a repetitive mild traumatic brain injury (rmTBI) mouse model and thus fits the pharmacological scope of </span><em>pharmanutrition.</em></p></div><div><h3>Methods</h3><p>Balb c mice were divided into four groups, sham, crocin sham, TBI and crocin TBI. Injured groups received seven multiple closed brain injuries. Treated groups were injected with crocin (30 mg/kg) 30 min before each hit. Brain cortices<span> were extracted 24 h post the last injury for molecular analysis. Brain cytokine levels of IL-6 and IL-10 were measured using ELISA. Also, using RT-PCR, the expression levels of the following genes, Bcl-2, caspase3, Bax, P53, NF-κB, Nrf2, HO-1 and NQO1 were assessed.</span></p></div><div><h3>Results</h3><p><span><span>There was a significant increase in the level of the inflammatory cytokine Il-6. Crocin administration induced a decrease in IL-6 accompanied with elevation in the anti-apoptotic cytokine IL-10. Crocin induced a decrease in the gene expression of the apoptotic factors caspase3, Bax and P53 in injured mice and enhanced the mRNA levels of the anti-apoptotic Bcl-2. Also, crocin enhanced the </span>gene expression levels of </span>transcription factor Nrf2<span> and the antioxidant enzymes HO-1 and NQO-1 whereas reduced the expression of NF-κB.</span></p></div><div><h3>Conclusion</h3><p>Crocin exerted its neuroprotective effect following rmTBI. Crocin proves to play a prospect role in conferring protection against concussions.</p></div>","PeriodicalId":20049,"journal":{"name":"PharmaNutrition","volume":"21 ","pages":"Article 100308"},"PeriodicalIF":2.4000,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"Crocin suppresses inflammation-induced apoptosis in rmTBI mouse model via modulation of Nrf2 transcriptional activity\",\"authors\":\"Marwa Salem, Mariam Shaheen, Jamilah Borjac\",\"doi\":\"10.1016/j.phanu.2022.100308\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background</h3><p><span><span>Repetitive mild traumatic brain injury (rmTBI) has been considered a serious health issue. </span>Crocin<span>, a bioactive carotenoid in </span></span><span><em>Crocus sativus</em></span><span> (saffron) is well known for its anti-inflammatory and anti-oxidant properties. The aim of this study is to investigate the neuroprotective role of crocin in a repetitive mild traumatic brain injury (rmTBI) mouse model and thus fits the pharmacological scope of </span><em>pharmanutrition.</em></p></div><div><h3>Methods</h3><p>Balb c mice were divided into four groups, sham, crocin sham, TBI and crocin TBI. Injured groups received seven multiple closed brain injuries. Treated groups were injected with crocin (30 mg/kg) 30 min before each hit. Brain cortices<span> were extracted 24 h post the last injury for molecular analysis. Brain cytokine levels of IL-6 and IL-10 were measured using ELISA. Also, using RT-PCR, the expression levels of the following genes, Bcl-2, caspase3, Bax, P53, NF-κB, Nrf2, HO-1 and NQO1 were assessed.</span></p></div><div><h3>Results</h3><p><span><span>There was a significant increase in the level of the inflammatory cytokine Il-6. Crocin administration induced a decrease in IL-6 accompanied with elevation in the anti-apoptotic cytokine IL-10. Crocin induced a decrease in the gene expression of the apoptotic factors caspase3, Bax and P53 in injured mice and enhanced the mRNA levels of the anti-apoptotic Bcl-2. Also, crocin enhanced the </span>gene expression levels of </span>transcription factor Nrf2<span> and the antioxidant enzymes HO-1 and NQO-1 whereas reduced the expression of NF-κB.</span></p></div><div><h3>Conclusion</h3><p>Crocin exerted its neuroprotective effect following rmTBI. Crocin proves to play a prospect role in conferring protection against concussions.</p></div>\",\"PeriodicalId\":20049,\"journal\":{\"name\":\"PharmaNutrition\",\"volume\":\"21 \",\"pages\":\"Article 100308\"},\"PeriodicalIF\":2.4000,\"publicationDate\":\"2022-09-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"PharmaNutrition\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2213434422000214\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"NUTRITION & DIETETICS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"PharmaNutrition","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2213434422000214","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"NUTRITION & DIETETICS","Score":null,"Total":0}
Crocin suppresses inflammation-induced apoptosis in rmTBI mouse model via modulation of Nrf2 transcriptional activity
Background
Repetitive mild traumatic brain injury (rmTBI) has been considered a serious health issue. Crocin, a bioactive carotenoid in Crocus sativus (saffron) is well known for its anti-inflammatory and anti-oxidant properties. The aim of this study is to investigate the neuroprotective role of crocin in a repetitive mild traumatic brain injury (rmTBI) mouse model and thus fits the pharmacological scope of pharmanutrition.
Methods
Balb c mice were divided into four groups, sham, crocin sham, TBI and crocin TBI. Injured groups received seven multiple closed brain injuries. Treated groups were injected with crocin (30 mg/kg) 30 min before each hit. Brain cortices were extracted 24 h post the last injury for molecular analysis. Brain cytokine levels of IL-6 and IL-10 were measured using ELISA. Also, using RT-PCR, the expression levels of the following genes, Bcl-2, caspase3, Bax, P53, NF-κB, Nrf2, HO-1 and NQO1 were assessed.
Results
There was a significant increase in the level of the inflammatory cytokine Il-6. Crocin administration induced a decrease in IL-6 accompanied with elevation in the anti-apoptotic cytokine IL-10. Crocin induced a decrease in the gene expression of the apoptotic factors caspase3, Bax and P53 in injured mice and enhanced the mRNA levels of the anti-apoptotic Bcl-2. Also, crocin enhanced the gene expression levels of transcription factor Nrf2 and the antioxidant enzymes HO-1 and NQO-1 whereas reduced the expression of NF-κB.
Conclusion
Crocin exerted its neuroprotective effect following rmTBI. Crocin proves to play a prospect role in conferring protection against concussions.