乳糜泻合并吸收不良综合征患者的蛋白质组学分析

IF 0.4 4区 医学 Q4 PHARMACOLOGY & PHARMACY
P. Gulbicka, Dagmara Pietkiewicz, Eliza Matuszewska, Szymon Plewa, Joanna Matysiak, M. Grzymisławski, Z. Kokot
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引用次数: 0

摘要

吸收不良综合征包括许多临床单位,导致慢性腹泻和营养不良。在乳糜泻和小肠细菌过度生长的情况下,诊断并不总是直截了当的,诊断通常需要结合血液抗体检测、肠道活检和特定基因检测。该研究旨在评估患有乳糜泻(n=31)、小肠细菌过度生长(n=28)和对照组(n=30)的患者的蛋白质/肽谱。人们还试图确定潜在的新诊断标记。适当制备后,使用MALDI-TOF质谱仪对多肽进行蛋白质组学分析。鉴定的蛋白为纤维蛋白原α链、激肽原-1、粘蛋白3A、补体C3、补体C4A和α -胰蛋白酶抑制剂间重链H4。进行的分析表明,一些蛋白质可能与所讨论疾病的存在有关,并证明蛋白质组学分析可能作为一种强大的诊断工具。由于乳糜泻和小肠细菌过度生长的诊断困难,显然需要进一步研究可能参与疾病过程的蛋白质的生物学作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Evaluation of the proteomic profile in patients with celiac disease and malabsorption syndrome
The malabsorption syndrome includes many clinical units that lead to chronic diarrhea and malnutrition. In celiac disease and small intestine bacterial overgrowth making the diagnosis is not always straightforward, and the diagnosis often requires combining blood antibody tests, intestinal biopsies, and specific genetic testing. The study aimed to assess patients' protein/peptide profiles with celiac disease (n=31), small intestinal bacterial overgrowth (n=28), and the control group (n=30). Attempts have also been made to identify potential new diagnostic markers. After appropriate preparation, a proteomic analysis of peptides was performed using the MALDI–TOF mass spectrometer. Identified proteins were fibrinogen alpha-chain, kininogen-1, mucin 3A, complement C3, complement C4A, and inter-alpha-trypsin inhibitor heavy chain H4. Performed analyses indicated some proteins that could be potentially involved in the presence of the disease in question and proved that proteomic profiling might serve as a powerful diagnostic tool. Due to the difficulties in celiac disease and small intestine bacterial overgrowth diagnosis, there is a clear need for further investigation of the biological role of proteins potentially involved in the course of the disease.
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来源期刊
CiteScore
0.80
自引率
0.00%
发文量
74
审稿时长
6-12 weeks
期刊介绍: The international journal of the Polish Pharmaceutical Society is published in 6 issues a year. The journal offers Open Access publication of original research papers, short communications and reviews written in English, in all areas of pharmaceutical sciences. The following areas of pharmaceutical sciences are covered: Analysis, Biopharmacy, Drug Biochemistry, Drug Synthesis, Natural Drugs, Pharmaceutical Technology, Pharmacology and General. A bimonthly appearing in English since 1994, which continues “Acta Poloniae Pharmaceutica”, whose first issue appeared in December 1937. The war halted the activity of the journal’s creators. Issuance of “Acta Poloniae Pharmaceutica” was resumed in 1947. From 1947 the journal appeared irregularly, initially as a quarterly, then a bimonthly. In the years 1963 – 1973 alongside the Polish version appeared the English edition of the journal. Starting from 1974 only works in English are published in the journal. Since 1995 the journal has been appearing very regularly in two-month intervals (six books a year). The journal publishes original works from all fields of pharmacy, summaries of postdoctoral dissertations and laboratory notes.
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