用质体标记解析capparate (Capparaceae)的物种复合体

IF 0.6 4区生物学 Q4 PLANT SCIENCES

Annales Botanici Fennici Pub Date : 2022-06-17 DOI:10.5735/085.059.0124

S. Maurya, R. Choudhary

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引用次数: 0

摘要

Capparis是被子植物Capparaceae中最大的属，约有142个分类群，具有很高的药用价值和经济价值。几个分类群，如软骨石斛、克莱霍氏石斛、白蜡石斛、月光石斛、刺石斛、刺石斛和刺石斛亚种。喜马拉雅不同的植物在形态上很难区分，它们的地理分布都有部分重叠。本研究评估了四个可塑性位点(rbcL, matK, trnL-trnF和psbA-trnH)在Capparis中分解三种物种复合物的潜力。成功的扩增和测序以及统计分析表明，matK, trnL-trnF及其组合是最有效的DNA标记。利用这些标记，我们能够区分从其自然栖息地和生药市场收集的不同Capparis分类群。基于形态学和分子数据，恢复了喜马拉雅羊草的种级。澄清了鲜为人知的C. danielii和C. inccanescens与C. sepiaria的系统发育关系。

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Species Complexes in Capparis (Capparaceae) Resolved with Plastidial Markers

Capparis with about 142 taxa is the largest genus in the angiosperm family Capparaceae, and is of high medicinal and economic importance. Several taxa such as C. cartilaginea, C. cleghornii, C. incanescens, C. moonii, C. roxburghii, C. sepiaria, C. spinosa and C. spinosa subsp. himalayensis are difficult to distinguish from each other based on morphology, and they all have partly overlapping geographic distributions. This study assesses the potential of four plastidial loci (rbcL, matK, trnL-trnF, and psbA-trnH) to resolve three species complexes in Capparis. Successful amplification and sequencing, as well as statistical analyses, revealed matK, trnL-trnF, and their combination to be the most effective DNA markers. With these markers we were able to distinguish different Capparis taxa collected from their natural habitats and from the crude drug market. The species rank of C. himalayensis was reinstated based on morphology and molecular data. The phylogenetic relationships of the poorly known C. danielii and C. incanescens with C. sepiaria were clarified.

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来源期刊

Annales Botanici Fennici 生物-植物科学

CiteScore

1.30

自引率

14.30%

发文量

审稿时长

6-12 weeks

期刊介绍： The journal publishes original, previously unpublished papers on plant and fungal: • systematics, evolution, phylogeography, taxonomy and nomenclature, • population biology and genetics, • physiology and molecular biology, • terrestrial and aquatic ecology (including effects of climate change), • ecology and reproduction of invasive species as well as their effects on native populations, • phytogeography and paleoecology.