Three different techniques to reconstruct 3D view of SEM images by using only free available software

In the present work, three different techniques were used to obtain a 3D view of the SEM images of adipose tissue samples taken from volunteer obese patients by only freely available software. The extrapolation of spatial data demonstrates the most promising results compared to the results of a stereoscopic view or interactive surface plots. The 3D reconstructed view of SEM images by extrapolation of spatial data allows one to obtain the depth of the sample from the SEM images. The reconstructed model also might be printed with a 3D printer, which extends the usage possibilities of this technique.