大戟核糖体DNA内部转录间隔序列的分子鉴定

IF 0.6 4区生物学 Q4 PLANT SCIENCES

Bangladesh Journal of Plant Taxonomy Pub Date : 2021-06-22 DOI:10.3329/bjpt.v28i1.54212

M. M. Alqahtani, M. A. Ali, M. Rahman, Fahad Al Hemaid, S. Kambhar, Joongku Lee

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引用次数: 0

摘要

核核糖体DNA（nrDNA）的内部转录间隔序列（ITS）由于长度较短，易于使用通用引物扩增，并且具有在较低分类水平上区分分类单元的辨别能力，因此在植物分子系统发育学中常用于基于分子的分类鉴定和DNA条形码。目前对ITS nrDNA序列的分子系统发育分析主要是为了确定沙特阿拉伯报道的大戟科一个尚未解决的医学重要物种大戟的分类地位。其整个ITS区的总长度为644个核苷酸。研究表明，schimperiana与Esula亚属的成员非常接近。孟加拉国植物分类学杂志。28（1）:125-1302021（6月）

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Molecular authentication of Euphorbia schimperiana Scheele using internal transcribed spacer sequences of nuclear ribosomal DNA

The Internal Transcribed Spacers (ITS) sequences of nuclear ribosomal DNA (nrDNA) are commonly used in plant molecular phylogenetics for the molecular based taxonomic identification and DNA barcoding because of shorter length and easy to amplify by using the universal primers, and further has discrimination ability to distinguish the taxon at lower taxonomic level. The present molecular phylogenetic analysis of ITS nrDNA sequences focuses to determine the taxonomic status of an unresolved medicinally important species Euphorbia schimperiana Scheele of the family Euphorbiaceae reported from Saudi Arabia. The combined length of the entire ITS region in E. schimperiana is 644 nucleotides. The study reveals that E. schimperiana shows a close proximity with the members of the subgenus Esula. Bangladesh J. Plant Taxon. 28(1): 125-130, 2021 (June)

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来源期刊

Bangladesh Journal of Plant Taxonomy 生物-植物科学

CiteScore

0.42

自引率

44.40%

发文量

审稿时长

6 months

期刊介绍： Bangladesh is a humid, subtropical country favouring luxuriant growth of microorganisms, fungi and plants from algae to angiosperms with rich diversity. She has the largest mangrove forest of the world in addition to diverse hilly and wetland habitats. More than a century back, foreign explorers endeavoured several floral expeditions, but little was done for non-vasculars and pteridophytes. In recent times, Bangladesh National Herbarium has been carrying out taxonomic research in Bangladesh along with few other national institutes (e.g. Department of Botany of public universities and Bangladesh Forest Research Institute).