益生菌与白藜芦醇联合治疗乳腺癌耐药的体内外研究

Q3 Medicine

Current Signal Transduction Therapy Pub Date : 2023-02-28 DOI:10.2174/1574362418666230228122251

Wamidh H. Talib, Faten Farhan

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引用次数: 0

摘要

为了评价新的针对乳腺癌耐药的抗癌组合，益生菌通过激活多种免疫机制在免疫系统中具有显著的活性。白藜芦醇是一种非类黄酮多酚。它具有多种药理作用，包括抗氧化、抗炎、抗增殖和抗血管生成。本研究探讨了益生菌与白藜芦醇联合治疗敏感和顺铂耐药乳腺癌的潜在免疫调节和抗肿瘤活性。在体内部分，荷瘤小鼠分别给予2.5*108 CFU/ml益生菌、50mg/kg白藜芦醇、益生菌与白藜芦醇联合、载药、顺铂治疗。用敏感的EMT6/P和顺铂耐药的EMT6/CPR癌细胞株接种Balb/C小鼠，观察其体内抗肿瘤活性。采用MTT法评价益生菌、白藜芦醇及其联合处理的抗增殖活性。采用LDH比色法测定各处理对自然杀伤细胞活性的影响。采用硝基蓝四氮唑法和中性红法评价巨噬细胞功能。联合治疗对两种细胞系的脾淋巴细胞增殖、巨噬细胞功能、吞噬功能和胞饮功能均有增强作用。结果表明，EMT6/P和EMT6/CPR小鼠的肿瘤大小和重量显著减少。益生菌和白藜芦醇的联合治疗对敏感和顺铂耐药的乳腺癌细胞具有重要的活性，并可能作为免疫系统的刺激物。因此，益生菌与白藜芦醇联合应用于癌症防治方面值得进一步研究。需要进一步的研究来充分了解这种组合的作用机制

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Combination of probiotics and resveratrol to target drug resistance in breast cancer: an in vitro and in vivo study

To evaluate new anticancer combination to target drug resistance in breast cancer Probiotics have significant activity in the immune system by the activation of multiple immune mechanisms. Resveratrol is a non-flavonoid polyphenol. It has various pharmacological effects, including anti-oxidative, anti-inflammatory, anti-proliferative, and anti-angiogenesis. The present study investigates the potential immunomodulatory and antitumor activity of the combination treatment of probiotics and resveratrol in sensitive and cisplatin resistant breast cancer. In the in vivo part, tumor-bearing mice received one of the following treatments: 2.5*108 CFU/ml probiotics, 50mg/kg resveratrol, the combination of probiotics and resveratrol, vehicle, or cisplatin. Balb/C mice were inoculated with sensitive EMT6/P & cisplatin resistance EMT6/CPR cancer cell lines and in vivo antitumor activity was evaluated. The antiproliferative activity of the probiotics, resveratrol, and their combination treatments was assessed using MTT assay to evaluate lymphocyte proliferation activity. LDH colorimetric assay was conducted to measure the effectiveness of the treatments on Natural killer cells activity. Nitro blue tetrazolium assay and neutral red method have been used to evaluate macrophage function. The combination treatment showed enhancement effect in splenic lymphocytes proliferation, macrophage function, phagocytosis, and pinocytosis in both cell lines. It was shown that a significant reduction in tumor size and weight in EMT6/P and EMT6/CPR bearing mice occurred. The combination treatment of probiotics and resveratrol had a valuable activity against sensitive and cisplatin resistant breast cancer cells and may act as a stimulator of the immune system. Therefore, the combination of probiotics and resveratrol deserves further analysis to be used in cancer prevention and treatment. Further studies are needed to fully understand the mechanisms of action of this combination

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来源期刊

Current Signal Transduction Therapy 医学-药学

CiteScore

1.70

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： In recent years a breakthrough has occurred in our understanding of the molecular pathomechanisms of human diseases whereby most of our diseases are related to intra and intercellular communication disorders. The concept of signal transduction therapy has got into the front line of modern drug research, and a multidisciplinary approach is being used to identify and treat signaling disorders. The journal publishes timely in-depth reviews, research article and drug clinical trial studies in the field of signal transduction therapy. Thematic issues are also published to cover selected areas of signal transduction therapy. Coverage of the field includes genomics, proteomics, medicinal chemistry and the relevant diseases involved in signaling e.g. cancer, neurodegenerative and inflammatory diseases. Current Signal Transduction Therapy is an essential journal for all involved in drug design and discovery.