{"title":"分光光度法和数字光度法测定化学需氧量的比较","authors":"Rhea Mallari, J. R. Micor, E. D. del Rosario","doi":"10.47125/jesam/2018_2/03","DOIUrl":null,"url":null,"abstract":"Visible spectrophotometry (VS) and digital photometry (DP) for determining chemical oxygen demand (COD) were compared; the latter method involved image processing of digital photographs of analyte solutions using RGB-AIC software. Statistical analysis showed that COD values of untreated and treated (trickling filter) wastewater samples from a hog slaughterhouse were not significantly different using VS and DP methods. The COD values were not significantly different among the treated samples as well using a* and L* plots. Tests for accuracy and repeatability of the DP method showed acceptable results. The calculated limit of detection (LOD) for DP was 0.73 mg L-1 while the LOD for VS was 0.33 mg L-1. The accuracy of the DP method was validated using glucose solutions of known COD values; t-tests performed at 95% confidence level showed no significant differences in COD values between (1) theory and experiment, (2) VS and DP and (3) a* and L* plots. These findings suggest that digital photometry is accurate and can be used as an equally accurate alternative to conventional spectrophotometry.","PeriodicalId":15657,"journal":{"name":"Journal of Environmental Science and Management","volume":" ","pages":""},"PeriodicalIF":0.4000,"publicationDate":"2019-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"Comparison of Spectrophotometric and Digital Photometric Methods for Determining Chemical Oxygen Demand\",\"authors\":\"Rhea Mallari, J. R. Micor, E. D. del Rosario\",\"doi\":\"10.47125/jesam/2018_2/03\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Visible spectrophotometry (VS) and digital photometry (DP) for determining chemical oxygen demand (COD) were compared; the latter method involved image processing of digital photographs of analyte solutions using RGB-AIC software. Statistical analysis showed that COD values of untreated and treated (trickling filter) wastewater samples from a hog slaughterhouse were not significantly different using VS and DP methods. The COD values were not significantly different among the treated samples as well using a* and L* plots. Tests for accuracy and repeatability of the DP method showed acceptable results. The calculated limit of detection (LOD) for DP was 0.73 mg L-1 while the LOD for VS was 0.33 mg L-1. The accuracy of the DP method was validated using glucose solutions of known COD values; t-tests performed at 95% confidence level showed no significant differences in COD values between (1) theory and experiment, (2) VS and DP and (3) a* and L* plots. These findings suggest that digital photometry is accurate and can be used as an equally accurate alternative to conventional spectrophotometry.\",\"PeriodicalId\":15657,\"journal\":{\"name\":\"Journal of Environmental Science and Management\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":0.4000,\"publicationDate\":\"2019-09-25\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Environmental Science and Management\",\"FirstCategoryId\":\"93\",\"ListUrlMain\":\"https://doi.org/10.47125/jesam/2018_2/03\",\"RegionNum\":4,\"RegionCategory\":\"环境科学与生态学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"ENVIRONMENTAL SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Environmental Science and Management","FirstCategoryId":"93","ListUrlMain":"https://doi.org/10.47125/jesam/2018_2/03","RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"ENVIRONMENTAL SCIENCES","Score":null,"Total":0}
Comparison of Spectrophotometric and Digital Photometric Methods for Determining Chemical Oxygen Demand
Visible spectrophotometry (VS) and digital photometry (DP) for determining chemical oxygen demand (COD) were compared; the latter method involved image processing of digital photographs of analyte solutions using RGB-AIC software. Statistical analysis showed that COD values of untreated and treated (trickling filter) wastewater samples from a hog slaughterhouse were not significantly different using VS and DP methods. The COD values were not significantly different among the treated samples as well using a* and L* plots. Tests for accuracy and repeatability of the DP method showed acceptable results. The calculated limit of detection (LOD) for DP was 0.73 mg L-1 while the LOD for VS was 0.33 mg L-1. The accuracy of the DP method was validated using glucose solutions of known COD values; t-tests performed at 95% confidence level showed no significant differences in COD values between (1) theory and experiment, (2) VS and DP and (3) a* and L* plots. These findings suggest that digital photometry is accurate and can be used as an equally accurate alternative to conventional spectrophotometry.
期刊介绍:
The Journal of Environmental Science and Management (JESAM) is an international scientific journal produced semi-annually by the University of the Philippines Los Baños (UPLB).
JESAM gives particular premium to manuscript submissions that employ integrated methods resulting to analyses that provide new insights in environmental science, particularly in the areas of:
environmental planning and management;
protected areas development, planning, and management;
community-based resources management;
environmental chemistry and toxicology;
environmental restoration;
social theory and environment; and
environmental security and management.