内源性大麻素及其代谢酶与自闭症谱系障碍儿童严重程度的关系

中华行为医学与脑科学杂志 Pub Date : 2020-03-20 DOI:10.3760/CMA.J.CN371468-20190905-00595

Dexin Li, Ling Li, M. Zou, Yu Liu, Shuang Xie, Caihong Sun

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引用次数: 0

摘要

目的探讨内源性大麻素（eCB）及其代谢酶与自闭症谱系障碍（ASD）严重程度的关系，为研究ASD的病因病机提供理论依据。方法采用病例对照研究方法，收集2017年12月至2018年12月在哈尔滨医科大学儿童发展行为研究中心和省级自闭症康复机构接受康复训练的58名自闭症谱系障碍儿童作为ASD组。根据性别和年龄1∶1匹配原则，选取黑龙江省58名正常儿童作为对照组，采用液相色谱-串联质谱（LC-MS/MS）和实时定量PCR（RT-qPCR）检测ASD组和对照组的eCB，油酰乙醇酰胺（OEA）及其代谢酶：n-酰基磷脂酰乙醇胺特异性磷脂酶D（NAPE-PLD）、脂肪酸酰胺水解酶（FAAH）、单酰基甘油脂肪酶（MAGL）和二酰基甘油脂酶（DAGL）mRNA表达水平。采用Pearson相关分析eCB水平与ASD儿童的严重程度。结果ASD患儿的AEA、OEA和PEA水平分别为（10.10±2.6）nmol/L、（24.30±5.60）nmol/L、（15.92±2.28）nmol/L，均低于对照组（（13.46±3.04）nmol、（27.85±6.89）nmol/L、（17.87±2.67）nmol／L，t=-6.612、-3.99、-4.779，结论ASD患儿的eCB及其代谢酶可能存在代谢异常，eCB水平与ASD的严重程度有关。关键词：自闭症谱系障碍；内源性大麻素；液相色谱-串联质谱法

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Association of endocannabinoid and its metabolic enzymes with severity in children with autism spectrum disorder

Objective To explore the relationship between endocannabinoid (eCB) and its metabolic enzymes and severity of autism spectrum disorder (ASD), and to provide a theoretical basis for the study of the etiology and pathogenesis of ASD. Methods A case-control study was conducted to collect 58 ASD children who underwent rehabilitation training at the Children's Developmental Behavior Research Center of Harbin Medical University and the provincial autism rehabilitation facility from December 2017 to December 2018 as the ASD group.According to the principle of gender and age 1∶1 matching, 58 normal children were selected as control group in Heilongjiang Province.Liquid chromatography-tandem mass spectrometry (LC-MS/MS) and real-time quantitative PCR (RT-qPCR) were used to detect eCB of ASD group and control group, including anandamide (AEA), 2-arachidonoylglycerol (2-AG), palmitoylethanolamide (PEA), oleoylethanolamide (OEA) and its metabolic enzymes: n-acylphosphatidylethanolamine-specific phospholipase D (NAPE-PLD), fatty acid amide hydrolase (FAAH), monoacylglycerol lipase (MAGL) and diacylglycerol lipase (DAGL) mRNA expression levels.Pearson correlation was used to analyze the level of eCB and ASD children's severity. Results The levels of AEA, OEA and PEA in ASD children ((10.10±2.6)nmol/L, (24.30±5.60)nmol/L, (15.92±2.28)nmol/L) were lower than those in the control group ((13.46±3.04)nmol/L, (27.85± 6.89)nmol/L, (17.87±2.67)nmol/L, t=-6.612, -3.99, -4.779, P 0.05). The level of PEA in the ASD group was negatively correlated with the total score of the autism behavior checklist (ABC) (r=-0.288, P<0.05). Conclusion There may be metabolic abnormalities in eCB and its metabolic enzymes in ASD children, and the level of eCB is related with the severity of ASD. Key words: Autism spectrum disorder; Endocannabinoid; Liquid chromatography-tandem mass spectrometry

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来源期刊

中华行为医学与脑科学杂志

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8131

期刊介绍： "Chinese Journal of Behavioral Medicine and Brain Science" (CN 37-1468/R, ISSN 1674-6554) is a national academic journal under the supervision of the National Health Commission, sponsored by the Chinese Medical Association and Jining Medical College. The journal was founded in June 1992 and was formerly known as "Chinese Journal of Behavioral Medicine" (1992-1993) and "Chinese Behavioral Medical Science" (1994-2008). In 2009, it was renamed "Chinese Journal of Behavioral Medicine and Brain Science" with the approval of the State Administration of Press, Publication, Radio, Film and Television. The purpose of "Chinese Journal of Behavioral Medicine and Brain Science" is to implement the health and health policies of the Party and the State, implement the principle of combining theory with practice and popularization and improvement, and reflect the major progress in the theory and practical application of behavioral medicine and brain science in my country. It publishes academic papers and scientific research results in the field of behavioral medicine and brain science in my country, and has columns such as monographs/reviews, basic research, clinical research, health prevention, methods and techniques, psychological behavior and evaluation, and systematic evaluation.