Kehinde Oluseun Sodeinde , Akinwunmi Oluwaseun Adeoye , Adedeji Adesipo , Adebayo A. Adeniyi , John Adeolu Falode , Tajudeen Olabisi Obafemi , Samuel Olalekan Olusanya , Linette Twigge , Jeanet Conradie , Timothy O. Mosaku
{"title":"异角鳗β-豆甾醇(含麦角甾醇)和木酸对线粒体通透性转移孔的分离、表征及调控作用","authors":"Kehinde Oluseun Sodeinde , Akinwunmi Oluwaseun Adeoye , Adedeji Adesipo , Adebayo A. Adeniyi , John Adeolu Falode , Tajudeen Olabisi Obafemi , Samuel Olalekan Olusanya , Linette Twigge , Jeanet Conradie , Timothy O. Mosaku","doi":"10.1016/j.chmed.2023.01.006","DOIUrl":null,"url":null,"abstract":"<div><h3>Objective</h3><p>Secondary metabolites and polyphenolic compounds from medicinal plants have been demonstrated to have multiple biological functions with promising research and development prospects. This study examined the effect of <em>β</em>-stigmasterol (with ergosterol) and xylopic acid isolated from <em>Anchomanes difformis</em> on liver mitochondrial permeability transition pore (mPTP).</p></div><div><h3>Methods</h3><p>The compounds were isolated by vacuum liquid chromatography. Mitochondrial swelling was assessed as changes in absorbance under succinate-energized conditions.</p></div><div><h3>Results</h3><p><sup>1</sup>H and <sup>13</sup>C NMR spectroscopic elucidation of the isolates affirmed the presence of <em>β</em>-stigmasterol with ergosterol (1:0.3) and xylopic acid. The isolates reversed the increase in lipid peroxidation and inhibited the opening of mitochondrial permeability transition pores caused by calcium and glucose. Pharmacological inhibition of mPTP offers a promising therapeutic target for the treatment of mitochondrial-associated disorders.</p></div><div><h3>Conclusion</h3><p>Reduction in the activity of calcium ATPase and the expression of Caspase-3 and −9 were observed, suggesting that they could play a role in protecting physicochemical properties of membrane bilayers from free radical-induced severe cellular damage and be useful in the management of diseases where much apoptosis occurs.</p></div>","PeriodicalId":9916,"journal":{"name":"Chinese Herbal Medicines","volume":"15 4","pages":"Pages 533-541"},"PeriodicalIF":4.7000,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1674638423000709/pdfft?md5=8756c81ae21b161363cbb5e50eb810a4&pid=1-s2.0-S1674638423000709-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Isolation, characterization and modulatory potentials of β-stigmasterol, ergosterol and xylopic acid from Anchomanes difformis on mitochondrial permeability transition pore in vitro\",\"authors\":\"Kehinde Oluseun Sodeinde , Akinwunmi Oluwaseun Adeoye , Adedeji Adesipo , Adebayo A. Adeniyi , John Adeolu Falode , Tajudeen Olabisi Obafemi , Samuel Olalekan Olusanya , Linette Twigge , Jeanet Conradie , Timothy O. Mosaku\",\"doi\":\"10.1016/j.chmed.2023.01.006\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Objective</h3><p>Secondary metabolites and polyphenolic compounds from medicinal plants have been demonstrated to have multiple biological functions with promising research and development prospects. This study examined the effect of <em>β</em>-stigmasterol (with ergosterol) and xylopic acid isolated from <em>Anchomanes difformis</em> on liver mitochondrial permeability transition pore (mPTP).</p></div><div><h3>Methods</h3><p>The compounds were isolated by vacuum liquid chromatography. Mitochondrial swelling was assessed as changes in absorbance under succinate-energized conditions.</p></div><div><h3>Results</h3><p><sup>1</sup>H and <sup>13</sup>C NMR spectroscopic elucidation of the isolates affirmed the presence of <em>β</em>-stigmasterol with ergosterol (1:0.3) and xylopic acid. The isolates reversed the increase in lipid peroxidation and inhibited the opening of mitochondrial permeability transition pores caused by calcium and glucose. Pharmacological inhibition of mPTP offers a promising therapeutic target for the treatment of mitochondrial-associated disorders.</p></div><div><h3>Conclusion</h3><p>Reduction in the activity of calcium ATPase and the expression of Caspase-3 and −9 were observed, suggesting that they could play a role in protecting physicochemical properties of membrane bilayers from free radical-induced severe cellular damage and be useful in the management of diseases where much apoptosis occurs.</p></div>\",\"PeriodicalId\":9916,\"journal\":{\"name\":\"Chinese Herbal Medicines\",\"volume\":\"15 4\",\"pages\":\"Pages 533-541\"},\"PeriodicalIF\":4.7000,\"publicationDate\":\"2023-10-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S1674638423000709/pdfft?md5=8756c81ae21b161363cbb5e50eb810a4&pid=1-s2.0-S1674638423000709-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Chinese Herbal Medicines\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1674638423000709\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, MEDICINAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Chinese Herbal Medicines","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1674638423000709","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, MEDICINAL","Score":null,"Total":0}
Isolation, characterization and modulatory potentials of β-stigmasterol, ergosterol and xylopic acid from Anchomanes difformis on mitochondrial permeability transition pore in vitro
Objective
Secondary metabolites and polyphenolic compounds from medicinal plants have been demonstrated to have multiple biological functions with promising research and development prospects. This study examined the effect of β-stigmasterol (with ergosterol) and xylopic acid isolated from Anchomanes difformis on liver mitochondrial permeability transition pore (mPTP).
Methods
The compounds were isolated by vacuum liquid chromatography. Mitochondrial swelling was assessed as changes in absorbance under succinate-energized conditions.
Results
1H and 13C NMR spectroscopic elucidation of the isolates affirmed the presence of β-stigmasterol with ergosterol (1:0.3) and xylopic acid. The isolates reversed the increase in lipid peroxidation and inhibited the opening of mitochondrial permeability transition pores caused by calcium and glucose. Pharmacological inhibition of mPTP offers a promising therapeutic target for the treatment of mitochondrial-associated disorders.
Conclusion
Reduction in the activity of calcium ATPase and the expression of Caspase-3 and −9 were observed, suggesting that they could play a role in protecting physicochemical properties of membrane bilayers from free radical-induced severe cellular damage and be useful in the management of diseases where much apoptosis occurs.
期刊介绍：
Chinese Herbal Medicines is intended to disseminate the latest developments and research progress in traditional and herbal medical sciences to researchers, practitioners, academics and administrators worldwide in the field of traditional and herbal medicines. The journal's international coverage ensures that research and progress from all regions of the world are widely included.
CHM is a core journal of Chinese science and technology. The journal entered into the ESCI database in 2017, and then was included in PMC, Scopus and other important international search systems. In 2019, CHM was successfully selected for the “China Science and Technology Journal Excellence Action Plan” project, which has markedly improved its international influence and industry popularity. CHM obtained the first impact factor of 3.8 in Journal Citation Reports (JCR) in 2023.
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