基于再生丝素蛋白的新型组织工程血管基质的体外研究

IF 1.1 Q4 MEDICINE, RESEARCH & EXPERIMENTAL
Sovremennye Tehnologii v Medicine Pub Date : 2023-01-01 Epub Date: 2023-07-28 DOI:10.17691/stm2023.15.4.04
E S Prokudina, E A Senokosova, L V Antonova, E O Krivkina, E A Velikanova, T N Akentieva, T V Glushkova, V G Matveeva, N A Kochergin
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引用次数: 0

摘要

该研究旨在制作一种基于再生蚕丝纤维蛋白(SF)的血管补片,并在体外实验中将其与含有血管内皮生长因子(PHBV/PCL/VEGF)的聚羟基丁酸酯/戊酸酯/聚己内酯和商用牛心包膜(XP)瓣进行比较,研究其物理和机械特性、生物相容性和基质特性:组织工程基质由电纺丝制成。研究了材料的表面结构、物理和机械特性、血液相容性(红细胞溶血、聚集、粘附和血小板接触材料后的活化)以及血管补片的基质特性(EA.hy926 细胞在材料上的粘附性、存活率和代谢活性):结果:基于 SF 的基质和基于 PHBV/PCL/VEGF 的组织工程补片的表面具有多孔和纤维结构,而 XP 皮瓣更致密、更均匀。SF 基质的物理和机械特性接近于原生血管。此外,组织工程补片还表现出很高的血液相容性,与商用 XP 皮瓣没有区别。EA.hy926内皮细胞的附着力、存活率和代谢活性也与之前开发的PHBV/PCL/VEGF基质和XP皮瓣一致,这表明SF基质具有无毒性和生物相容性:结论:由再生 SF 制成的基质显示出令人满意的效果,与 PHBV/PCL/VEGF 和商用 XP 皮瓣的效果相当,在血小板粘附和活化方面,它们优于这些补片。总之,SF 可以被定义为具有足够生物相容性的材料,因此可以认为用它制成的组织工程基质有望植入血管壁。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
New Tissue-Engineered Vascular Matrix Based on Regenerated Silk Fibroin: in vitro Study.

The aim of the study was to make a vascular patch based on regenerated silk fibroin (SF) and study its physical and mechanical characteristics, biocompatibility and matrix properties in comparison with polyhydroxybutyrate/valerate/polycaprolactone with incorporated vascular endothelial growth factor (PHBV/PCL/VEGF) and commercial bovine xenopericardium (XP) flap in experiments in vitro.

Materials and methods: Tissue-engineered matrices were produced by electrospinning. The surface structure, physical and mechanical characteristics, hemocompatibility (erythrocyte hemolysis, aggregation, adhesion and activation of platelets after contact with the material) and matrix properties of vascular patches (adhesion, viability, metabolic activity of EA.hy926 cells on the material) were studied.

Results: The surface of SF-based matrices and PHBV/PCL/VEGF-based tissue engineered patches had a porous and fibrous structure compared to a denser and more uniform XP flap. The physical and mechanical characteristics of SF matrices were close to those of native vessels. Along with this, tissue-engineered patches demonstrated high hemocompatible properties, which do not differ from those for commercial XP flap. Adhesion, viability, and metabolic activity of EA.hy926 endothelial cells also corresponded to the previously developed PHBV/PCL/VEGF matrix and XP flap, which indicates the nontoxicity and biocompatibility of SF matrices.

Conclusion: Matrices produced from regenerated SF demonstrated satisfactory results, comparable to those for PHBV/PCL/VEGF and commercial XP flap, and in the case of platelet adhesion and activation, they outperformed these patches. In total, SF can be defined as material having sufficient biological compatibility, which makes it possible to consider a tissue-engineered matrix made from it as promising for implantation into the vascular wall.

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来源期刊
Sovremennye Tehnologii v Medicine
Sovremennye Tehnologii v Medicine MEDICINE, RESEARCH & EXPERIMENTAL-
CiteScore
1.80
自引率
0.00%
发文量
38
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