HPLC法同时测定妇科养容丸中多种成分的含量

IF 1.7 4区化学 Q3 CHEMISTRY, ANALYTICAL

Acta Chromatographica Pub Date : 2022-09-13 DOI:10.1556/1326.2022.01081

Hua Wang, Changjuan Zhan, Yi Wang

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引用次数: 0

摘要

福科养荣丸是一种传统的中成药，具有滋补气血、疏肝解郁、调经化瘀的功效，由16种中草药组成。为控制质量，建立了同时测定妇科养荣丸中芍药苷、橙皮苷和藁本内酯含量的高效液相色谱法。以乙腈-0.1%甲酸为流动相，采用Agilent ZORBAX Eclipse Plus C18色谱柱(250 mm × 4.6 mm, 5.0 μm)，流速1.0 mL min - 1梯度洗脱。芍药苷的检测波长为230 nm，橙皮苷和藁本内酯的检测波长为280 nm。温度是30°C。峰面积与各待测成分浓度在线性范围内呈良好的线性关系(r≥0.9994)。平均加样回收率为98.6% ~ 102.6%，rsd≤2.93%。结果表明，该方法准确、简便，适用于妇科养荣丸的质量控制。

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Simultaneous determination of multiple components in Fuke Yangrong pill by HPLC

Fuke Yangrong pill, a traditional Chinese patent medicine, with the functions of nourishing qi and blood, soothing liver and relieving depression, regulating menstruation and removing blood stasis, is composed of 16 Chinese medicinal herbs. For quality control purpose, an HPLC method was established for simultaneous quantification of paeoniflorin, hesperidin and ligustilide in Fuke Yangrong pill. With acetonitrile-0.1% formic acid as mobile phase, gradient elution was carried out using Agilent ZORBAX Eclipse Plus C18 column (250 mm × 4.6 mm, 5.0 μm) at 1.0 mL min−1. Detection wavelength was set at 230 nm for paeoniflorin, 280 nm for hesperidin and ligustilide. The temperature was 30 °C. There was a good linearity between the peak area and the concentration of each component to be measured within their linear range (r ≥ 0.9994). The average recoveries were between 98.6% and 102.6% with RSDs no more than 2.93%. This method was validated to be accurate and convenient, which is suitable for the quality control of Fuke Yangrong pill.

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来源期刊

Acta Chromatographica 化学-分析化学

CiteScore

4.00

自引率

0.00%

发文量

审稿时长

2.3 months

期刊介绍： Acta Chromatographica Open Access Acta Chromatographica publishes peer-reviewed scientific articles on every field of chromatography, including theory of chromatography; progress in synthesis and characterization of new stationary phases; chromatography of organic, inorganic and complex compounds; enantioseparation and chromatography of chiral compounds; applications of chromatography in biology, pharmacy, medicine, and food analysis; environmental applications of chromatography; analytical and physico-chemical aspects of sample preparation for chromatography; hyphenated and combined techniques; chemometrics and its applications in separation science.