不同溶剂提取的苦参叶和果实的化学成分:LC-MS鉴定、定量及降糖作用

IF 2.2 4区 农林科学 Q2 HORTICULTURE
Ebru Aydın
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引用次数: 0

摘要

宽叶七弦草(Phillyrea latifolia L.)是木犀科的一种灌木林,被广泛称为假女贞。本研究的目的是比较和评估宽叶P.latifolia L.果实和叶片的乙酸乙酯、甲醇和水提取物的植物化学成分、抗氧化和抗糖尿病活性。通过使用LCMS-2020四极质谱仪检测单个生物活性化合物并计算总酚含量(TPC)来分析酚类化合物。首次用DPPH自由基清除法测定了叶片和果实的抗氧化和抗糖尿病活性。水提取物比乙酸乙酯和甲醇提取物具有更高的抗氧化活性。在乙酸乙酯(854μg·g-1和1098μg·g–1)、甲醇(1241μg·g-1和2136.43μg·g/1)和水性(509μg·g.1和898.23μg•g-1)提取物中,检测到果实和叶片不同提取物中的单独成分为木犀草素-7-O-葡萄糖苷。乙酸乙酯和甲醇提取物对人类唾液α-淀粉酶的抑制活性比模拟女贞两部分的水提取物更强。类似地,从模拟女贞的叶子和果实中提取乙酸乙酯,在抑制α-葡萄糖苷酶活性方面分别比甲醇和水提取物表现出更好的抑制活性。这项研究表明,模拟女贞的果实和叶片都可以作为天然生物分子的潜在来源来促进健康活动。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Phytochemicals from Phillyrea latifolia L. leaves and fruit extracted with various solvents: Their identification and quantification by LC-MS and antihyperglycemic effects
ABSTRACT Phillyrea latifolia L. is a type of shrubland, which is widely known as mock privet, and belongs to the Oleaceae family. The objective of this study was to compare and assess the phytochemical composition, antioxidant and antidiabetic activities of ethyl acetate, methanol and aqueous extracts of the fruit and leaves of P. latifolia L. Phenolics were analysed by detecting individual bioactive compounds using an LCMS-2020 quadrupole mass spectrometer and by calculating total phenolic content (TPC). For the first time, the antioxidant and antidiabetic activities of both leaves and fruit were determined using DPPH radical scavenging. The aqueous extract was indicated to have higher antioxidant activities than ethyl acetate and methanol extracts. The individual constituents within the different extracts for both fruit and leaves were detected as the luteolin-7-O-glucoside in the ethyl acetate (854 μg · g–1 and 1,098 μg · g–1), methanol (1,241 μg · g–1 and 2,136.43 μg · g–1) and aqueous (509 μg · g–1 and 898.23 μg · g–1) extracts, respectively. Extractions of ethyl acetate and methanol demonstrated stronger inhibitory activity against human salivary α-amylase than the aqueous extract of both parts of the mock privet. Similarly, extraction of ethyl acetate from the leaves and fruit of the mock privet indicated significantly better inhibitory activity than the methanol and aqueous extracts, respectively, for the inhibition of α-glucosidase activity. This study indicates that both fruit and leaves of mock privet may use as a potential source of natural biomolecules to promote healthy activities.
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来源期刊
Folia Horticulturae
Folia Horticulturae Agricultural and Biological Sciences-Horticulture
CiteScore
3.40
自引率
0.00%
发文量
13
审稿时长
16 weeks
期刊介绍: Folia Horticulturae is an international, scientific journal published in English. It covers a broad research spectrum of aspects related to horticultural science that are of interest to a wide scientific community and have an impact on progress in both basic and applied research carried out with the use of horticultural crops and their products. The journal’s aim is to disseminate recent findings and serve as a forum for presenting views as well as for discussing important problems and prospects of modern horticulture, particularly in relation to sustainable production of high yield and quality of horticultural products, including their impact on human health.
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