{"title":"不同溶剂提取的苦参叶和果实的化学成分:LC-MS鉴定、定量及降糖作用","authors":"Ebru Aydın","doi":"10.2478/fhort-2023-0018","DOIUrl":null,"url":null,"abstract":"ABSTRACT Phillyrea latifolia L. is a type of shrubland, which is widely known as mock privet, and belongs to the Oleaceae family. The objective of this study was to compare and assess the phytochemical composition, antioxidant and antidiabetic activities of ethyl acetate, methanol and aqueous extracts of the fruit and leaves of P. latifolia L. Phenolics were analysed by detecting individual bioactive compounds using an LCMS-2020 quadrupole mass spectrometer and by calculating total phenolic content (TPC). For the first time, the antioxidant and antidiabetic activities of both leaves and fruit were determined using DPPH radical scavenging. The aqueous extract was indicated to have higher antioxidant activities than ethyl acetate and methanol extracts. The individual constituents within the different extracts for both fruit and leaves were detected as the luteolin-7-O-glucoside in the ethyl acetate (854 μg · g–1 and 1,098 μg · g–1), methanol (1,241 μg · g–1 and 2,136.43 μg · g–1) and aqueous (509 μg · g–1 and 898.23 μg · g–1) extracts, respectively. Extractions of ethyl acetate and methanol demonstrated stronger inhibitory activity against human salivary α-amylase than the aqueous extract of both parts of the mock privet. Similarly, extraction of ethyl acetate from the leaves and fruit of the mock privet indicated significantly better inhibitory activity than the methanol and aqueous extracts, respectively, for the inhibition of α-glucosidase activity. This study indicates that both fruit and leaves of mock privet may use as a potential source of natural biomolecules to promote healthy activities.","PeriodicalId":12277,"journal":{"name":"Folia Horticulturae","volume":null,"pages":null},"PeriodicalIF":2.2000,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Phytochemicals from Phillyrea latifolia L. leaves and fruit extracted with various solvents: Their identification and quantification by LC-MS and antihyperglycemic effects\",\"authors\":\"Ebru Aydın\",\"doi\":\"10.2478/fhort-2023-0018\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"ABSTRACT Phillyrea latifolia L. is a type of shrubland, which is widely known as mock privet, and belongs to the Oleaceae family. The objective of this study was to compare and assess the phytochemical composition, antioxidant and antidiabetic activities of ethyl acetate, methanol and aqueous extracts of the fruit and leaves of P. latifolia L. Phenolics were analysed by detecting individual bioactive compounds using an LCMS-2020 quadrupole mass spectrometer and by calculating total phenolic content (TPC). For the first time, the antioxidant and antidiabetic activities of both leaves and fruit were determined using DPPH radical scavenging. The aqueous extract was indicated to have higher antioxidant activities than ethyl acetate and methanol extracts. The individual constituents within the different extracts for both fruit and leaves were detected as the luteolin-7-O-glucoside in the ethyl acetate (854 μg · g–1 and 1,098 μg · g–1), methanol (1,241 μg · g–1 and 2,136.43 μg · g–1) and aqueous (509 μg · g–1 and 898.23 μg · g–1) extracts, respectively. Extractions of ethyl acetate and methanol demonstrated stronger inhibitory activity against human salivary α-amylase than the aqueous extract of both parts of the mock privet. Similarly, extraction of ethyl acetate from the leaves and fruit of the mock privet indicated significantly better inhibitory activity than the methanol and aqueous extracts, respectively, for the inhibition of α-glucosidase activity. This study indicates that both fruit and leaves of mock privet may use as a potential source of natural biomolecules to promote healthy activities.\",\"PeriodicalId\":12277,\"journal\":{\"name\":\"Folia Horticulturae\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.2000,\"publicationDate\":\"2023-06-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Folia Horticulturae\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.2478/fhort-2023-0018\",\"RegionNum\":4,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"HORTICULTURE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Folia Horticulturae","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.2478/fhort-2023-0018","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"HORTICULTURE","Score":null,"Total":0}
Phytochemicals from Phillyrea latifolia L. leaves and fruit extracted with various solvents: Their identification and quantification by LC-MS and antihyperglycemic effects
ABSTRACT Phillyrea latifolia L. is a type of shrubland, which is widely known as mock privet, and belongs to the Oleaceae family. The objective of this study was to compare and assess the phytochemical composition, antioxidant and antidiabetic activities of ethyl acetate, methanol and aqueous extracts of the fruit and leaves of P. latifolia L. Phenolics were analysed by detecting individual bioactive compounds using an LCMS-2020 quadrupole mass spectrometer and by calculating total phenolic content (TPC). For the first time, the antioxidant and antidiabetic activities of both leaves and fruit were determined using DPPH radical scavenging. The aqueous extract was indicated to have higher antioxidant activities than ethyl acetate and methanol extracts. The individual constituents within the different extracts for both fruit and leaves were detected as the luteolin-7-O-glucoside in the ethyl acetate (854 μg · g–1 and 1,098 μg · g–1), methanol (1,241 μg · g–1 and 2,136.43 μg · g–1) and aqueous (509 μg · g–1 and 898.23 μg · g–1) extracts, respectively. Extractions of ethyl acetate and methanol demonstrated stronger inhibitory activity against human salivary α-amylase than the aqueous extract of both parts of the mock privet. Similarly, extraction of ethyl acetate from the leaves and fruit of the mock privet indicated significantly better inhibitory activity than the methanol and aqueous extracts, respectively, for the inhibition of α-glucosidase activity. This study indicates that both fruit and leaves of mock privet may use as a potential source of natural biomolecules to promote healthy activities.
期刊介绍:
Folia Horticulturae is an international, scientific journal published in English. It covers a broad research spectrum of aspects related to horticultural science that are of interest to a wide scientific community and have an impact on progress in both basic and applied research carried out with the use of horticultural crops and their products. The journal’s aim is to disseminate recent findings and serve as a forum for presenting views as well as for discussing important problems and prospects of modern horticulture, particularly in relation to sustainable production of high yield and quality of horticultural products, including their impact on human health.