反相高效液相色谱法定量测定固定剂量复合纳米凝胶中6-姜辣素、e -谷固酮和z -谷固酮的含量

Q4 Pharmacology, Toxicology and Pharmaceutics
M. Momin, Vivek Basudkar, Sankalp Gharat, Anita Chando, T. Khan
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引用次数: 0

摘要

多草药配方已被证明对各种疾病的治疗有效,然而,开发有效的定量分析方法是一个主要挑战。本项目旨在建立一种反相高效液相色谱法(RP-HPLC)测定纳米乳凝胶中6-姜辣素(6-GIN)、e -谷胱甘酮(E-GGS)和z -谷胱甘酮(Z-GGS)含量的简便分析方法。以乙腈:水:甲醇(70:20:10 V/V/V)为流动相,1.0 mL min-1流速,光电二极管阵列检测6-GIN、E-GGS和Z-GGS。根据ICH Q2 (R1)指南对所开发的方法进行了线性、准确度、精密度、特异性和稳健性验证。制备的纳米凝胶中三种活性物质的药物含量在90% ~ 110% w/w之间。所建立的分析方法简便,可用于含有这些活性物质的固定剂量产品中6-GIN、E-GGS和Z-GGS的定量分析。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
QUANTITATIVE ESTIMATION OF 6-GINGEROL, E-GUGGULSTERONE AND Z-GUGGULSTERONE IN A FIXED DOSE COMBINATION NANOEMULGEL BY RP-HPLC
Polyherbal formulations have proved to be efficacious for the therapeutic treatment of various diseases, However, the development of validated robust analytical methods for quantification is a major challenge. The aim of this project was to develop a simple analytical method for the quantification of 6-gingerol (6-GIN), E-guggulsterone (E-GGS) and Z-guggulsterone (Z-GGS) in nanoemulsion based gel using reverse phase high performance liquid chromatography (RP-HPLC). 6-GIN, E-GGS and Z-GGS were quantified using acetonitrile: water: methanol (70:20:10 V/V/V) as the mobile phase at 1.0 mL min-1 flow rate with photodiode array detection. The developed method was validated for linearity, accuracy, precision, specificity and robustness as per ICH Q2 (R1) guidelines. The drug content of the three actives in the developed nanoemulgel was found to be between 90% to 110% w/w. The developed analytical method is simple and can be used for quantification of 6-GIN, E-GGS and Z-GGS in fixed dose product containing these actives.
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来源期刊
INDIAN DRUGS
INDIAN DRUGS Pharmacology, Toxicology and Pharmaceutics-Pharmaceutical Science
CiteScore
0.30
自引率
0.00%
发文量
98
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