银杏叶提取物对脑缺血模型大鼠大脑皮层氨基酸水平的影响

IF 2 4区 医学 Q2 INTEGRATIVE & COMPLEMENTARY MEDICINE
Cui Yiran , Wu Hongwei , Liu Mengting , Qin Haijiao , Liu Xin , Yang Hongjun
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引用次数: 0

摘要

目的探讨银杏叶提取物对大脑中动脉闭塞(MCAO)致脑缺血模型大鼠大脑皮层氨基酸水平的影响。方法采用MCAO建立大鼠脑缺血模型。将雄性大鼠分为阴性对照组(control)、假手术组(Sham)、缺血组(MCAO)和银杏叶提取物缺血组(MCAO_D)。各组均分为2个亚组,阻断时间分别为12和24 h。采用三重四极柱-液相色谱-质谱联用法定量测定大鼠大脑皮层中18种内源氨基酸的含量。结果与MCAO组比较,MCAO_D组大鼠行为表现、神经功能缺损评分、脑梗死体积均显著改善(P <0.05, P <0.01)。与假手术组比较,MCAO组大鼠大脑皮层17种氨基酸水平明显改变。丙氨酸(Ala)、异亮氨酸(Ile)、谷氨酸(Glu)、丝氨酸(Ser)、缬氨酸(Val)、苯丙氨酸(Phe)、脯氨酸(Pro)、苏氨酸(Thr)、赖氨酸(Lys)、酪氨酸(Tyr)、羟脯氨酸(Hyp)、精氨酸(Arg)、亮氨酸(Leu)、色氨酸(Trp)和甘氨酸(Gly)水平均升高(P <0.001, P <0.05), Gln和Tau水平降低(P <0.001, P <0.05)。与MCAO组比较,MCAO_D组银杏叶提取物在12 h和24 h显著下调了11种氨基酸的水平,尤其是精氨酸、苏氨酸和丝氨酸的水平。结论注射银杏叶提取物通过作用于大脑皮层的氨基酸对MCAO诱导的脑缺血模型大鼠有治疗作用。这种作用可能与调节大脑皮层的氨基酸代谢有关。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effect of Ginkgo biloba leaf extract on cerebral cortex amino acid levels in cerebral ischemia model rats

OBJECTIVE

To investigate the effect of Ginkgo biloba leaf extract on amino acid levels in the cerebral cortex of cerebral ischemia model rats induced by middle cerebral artery occlusion (MCAO).

METHODS

A rat model of cerebral ischemia was established by MCAO. Male rats were divided into a negative control group (Control), a sham-operated group (Sham), an ischemic group (MCAO), and an ischemic group treated with Ginkgo biloba leaf extract (MCAO_D). All groups were divided into two subgroups with occlusion times of 12 and 24 h, respectively. The levels of 18 endogenous amino acids in the cerebral cortex were quantified by triple quadrupole-liquid chromatography-mass spectrometry.

RESULTS

Compared with the MCAO group, behavioral performance, neurological deficit score, and cerebral infarct volume were significantly improved in the MCAO_D group (P < 0.05, P < 0.01). Compared with the sham group, the levels of 17 amino acids in the cerebral cortex were markedly changed in the MCAO group. The levels of Alanine (Ala), Isoleucine (Ile), Glutamic acid (Glu), Serine (Ser), Valine (Val), Phenylalanine (Phe), Proline (Pro), Threonine (Thr), Lysine (Lys), Tyrosine (Tyr), Hydroxyproline (Hyp), Arginine (Arg), Leucine (Leu), Tryptophan (Trp), and Glycine (Gly) were increased (P < 0.001, P < 0.05), while levels of Gln and Tau were decreased (P < 0.001, P < 0.05). Compared with the MCAO group, Ginkgo biloba extract treatment in the MCAO_D group significantly down-regulated the levels of 11 amino acids, especially those of Arg, Thr, and Ser in 12 or 24 h.

CONCLUSION

Injection of Ginkgo biloba leaf extract has a therapeutic effect on model rats with MCAO-induced cerebral ischemia by acting on amino acids in the cerebral cortex. This effect might be associated with the regulation of amino acid metabolism in the cerebral cortex.

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来源期刊
Journal of Traditional Chinese Medicine
Journal of Traditional Chinese Medicine INTEGRATIVE & COMPLEMENTARY MEDICINE-
CiteScore
2.40
自引率
3.80%
发文量
32269
审稿时长
2 months
期刊介绍: Journal of Traditional Chinese Medicine(JTCM) is devoted to clinical and theortical research on the use of acupuncture and Oriental medicine. The main columns include Clinical Observations, Basic Investigations, Reviews, Questions and Answers, an Expert''s Forum, and Discussions of Clinical Cases. Its key topics include acupuncture and electro-acupuncture, herbal medicine, homeopathy, masseotherapy, mind-body therapies, palliative care, and other CAM therapies.
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