Comfort O. Oladejo, Omowumi O. Ogundele, Abimbola R. Adeoti, Jesutola R. Atilola, M. Tolulope Olaleye, Afolabi C. Akinmoladun
{"title":"在锂匹罗卡品癫痫持续状态模型中，四翅目四胸膜减少氧化和促炎生化事件","authors":"Comfort O. Oladejo, Omowumi O. Ogundele, Abimbola R. Adeoti, Jesutola R. Atilola, M. Tolulope Olaleye, Afolabi C. Akinmoladun","doi":"10.1007/s13596-022-00675-x","DOIUrl":null,"url":null,"abstract":"<div><p><i>Tetrapleura tetraptera</i> Taub. (Fabaceae), commonly known as Aidan is ethnopharmacologically used for the management of health conditions such as diabetes mellitus, leprosy, epilepsy, and stroke in Nigeria. This study evaluated the anti-oxidoinflammatory properties of <i>T. tetraptera</i> methanol leaf extract (TTE) in lithium-pilocarpine-induced status epilepticus in Wistar rats. The extract was phytochemically screened and HPLC fingerprinting was performed. Animals were intraperitoneally administered with 127 mg/kg lithium chloride followed by 25 mg/kg pilocarpine 20 h later to induce status epilepticus. The animals were post treated with 50, 100 and 200 mg/kg TTE with 10 mg/kg valproic acid as the reference standard drug. Phytochemical screening of TTE confirmed the presence of tannins, phenols, alkaloids, terpenoids, flavonoids, saponins, glycosides and steroids. Quantitative phytochemical and antioxidant analyses of the extract indicated significant in vitro antioxidant and radical scavenging activity. HPLC analysis of the extract confirmed the presence of aridanin and polyphenols. TTE ameliorated redox imbalance by increasing markers for oxidative stress such as ferric reducing antioxidant power, glutathione level, catalase, glutathione S-transferase and superoxide dismutase activities. Moreover, TTE ameliorated pro-inflammatory events by reducing the level of the pro-inflammatory mediator nitric oxide, attenuating lipid peroxidation (which produces inflammatory lipid peroxidation-derived aldehydes), and decreasing the activities of xanthine oxidase and lactate dehydrogenase in the brain. These results indicated that the leaf of <i>Tetrapleura tetraptera</i> has therapeutic potential against status epilepticus by reversing oxidoinflammatory events. <i>Tetrapleura tetraptera</i> leaf extracts could be used to produce novel plant-based pharmaceuticals for treating status epilepticus and associated disorders.</p><h3>Graphic abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"23 4","pages":"1209 - 1220"},"PeriodicalIF":1.8000,"publicationDate":"2022-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Tetrapleura tetraptera curtails oxidative and proinflammatory biochemical events in lithium-pilocarpine model of status epilepticus\",\"authors\":\"Comfort O. Oladejo, Omowumi O. Ogundele, Abimbola R. Adeoti, Jesutola R. Atilola, M. Tolulope Olaleye, Afolabi C. Akinmoladun\",\"doi\":\"10.1007/s13596-022-00675-x\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p><i>Tetrapleura tetraptera</i> Taub. (Fabaceae), commonly known as Aidan is ethnopharmacologically used for the management of health conditions such as diabetes mellitus, leprosy, epilepsy, and stroke in Nigeria. This study evaluated the anti-oxidoinflammatory properties of <i>T. tetraptera</i> methanol leaf extract (TTE) in lithium-pilocarpine-induced status epilepticus in Wistar rats. The extract was phytochemically screened and HPLC fingerprinting was performed. Animals were intraperitoneally administered with 127 mg/kg lithium chloride followed by 25 mg/kg pilocarpine 20 h later to induce status epilepticus. The animals were post treated with 50, 100 and 200 mg/kg TTE with 10 mg/kg valproic acid as the reference standard drug. Phytochemical screening of TTE confirmed the presence of tannins, phenols, alkaloids, terpenoids, flavonoids, saponins, glycosides and steroids. Quantitative phytochemical and antioxidant analyses of the extract indicated significant in vitro antioxidant and radical scavenging activity. HPLC analysis of the extract confirmed the presence of aridanin and polyphenols. TTE ameliorated redox imbalance by increasing markers for oxidative stress such as ferric reducing antioxidant power, glutathione level, catalase, glutathione S-transferase and superoxide dismutase activities. Moreover, TTE ameliorated pro-inflammatory events by reducing the level of the pro-inflammatory mediator nitric oxide, attenuating lipid peroxidation (which produces inflammatory lipid peroxidation-derived aldehydes), and decreasing the activities of xanthine oxidase and lactate dehydrogenase in the brain. 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Tetrapleura tetraptera curtails oxidative and proinflammatory biochemical events in lithium-pilocarpine model of status epilepticus
Tetrapleura tetraptera Taub. (Fabaceae), commonly known as Aidan is ethnopharmacologically used for the management of health conditions such as diabetes mellitus, leprosy, epilepsy, and stroke in Nigeria. This study evaluated the anti-oxidoinflammatory properties of T. tetraptera methanol leaf extract (TTE) in lithium-pilocarpine-induced status epilepticus in Wistar rats. The extract was phytochemically screened and HPLC fingerprinting was performed. Animals were intraperitoneally administered with 127 mg/kg lithium chloride followed by 25 mg/kg pilocarpine 20 h later to induce status epilepticus. The animals were post treated with 50, 100 and 200 mg/kg TTE with 10 mg/kg valproic acid as the reference standard drug. Phytochemical screening of TTE confirmed the presence of tannins, phenols, alkaloids, terpenoids, flavonoids, saponins, glycosides and steroids. Quantitative phytochemical and antioxidant analyses of the extract indicated significant in vitro antioxidant and radical scavenging activity. HPLC analysis of the extract confirmed the presence of aridanin and polyphenols. TTE ameliorated redox imbalance by increasing markers for oxidative stress such as ferric reducing antioxidant power, glutathione level, catalase, glutathione S-transferase and superoxide dismutase activities. Moreover, TTE ameliorated pro-inflammatory events by reducing the level of the pro-inflammatory mediator nitric oxide, attenuating lipid peroxidation (which produces inflammatory lipid peroxidation-derived aldehydes), and decreasing the activities of xanthine oxidase and lactate dehydrogenase in the brain. These results indicated that the leaf of Tetrapleura tetraptera has therapeutic potential against status epilepticus by reversing oxidoinflammatory events. Tetrapleura tetraptera leaf extracts could be used to produce novel plant-based pharmaceuticals for treating status epilepticus and associated disorders.
期刊介绍：
Advances in Traditional Medicine (ADTM) is an international and peer-reviewed journal and publishes a variety of articles including original researches, reviews, short communications, and case-reports. ADTM aims to bridging the gap between Traditional knowledge and medical advances. The journal focuses on publishing valid, relevant, and rigorous experimental research and clinical applications of Traditidnal Medicine as well as medical classics. At the same time, the journal is devoted to communication among basic researcher and medical clinician interested in the advancement of Traditional Medicine. Topics covered by the journal are: Medical Classics & History; Biomedical Research; Pharmacology & Toxicology of Natural Products; Acupuncture & Moxibustion; Sasang Constitutional Medicine; Diagnostics and Instrumental Development; Clinical Research. ADTM is published four times yearly. The publication date of this journal is 30th March, June, September, and December.
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