露地和室内生长的木犀（库克）的植物化学筛选及其抗菌活性

IF 1 Q3 MULTIDISCIPLINARY SCIENCES

gazi university journal of science Pub Date : 2022-10-11 DOI:10.35378/gujs.995742

Nurkhalida Kamal, Hazniza Adnan, J. Latip

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引用次数: 0

摘要

木犀菌（库克）被称为虎奶蘑菇，是一种罕见而珍贵的药用蘑菇，在东南亚和华南地区广泛用于治疗多种疾病。本研究筛选了生长在野外和室内两种不同环境中的L.rhinocerotis（Cooke）Ryvarden菌核中的植物化学物质，并对其抗菌活性进行了评价。在本研究中，采用各种化学测试方法对犀核菌（库克）的乙酸乙酯和甲醇提取物进行了植物化学筛选，以鉴定提取物中存在的化合物。然后使用圆盘扩散法测试所有提取物在20和30mg/mL浓度下对三种不同细菌（包括金黄色葡萄球菌、大肠杆菌和铜绿假单胞菌）的抗菌活性。根据植物化学筛选结果，L.rhinocerotis（Cooke）Ryvarden的提取物显示存在类固醇、萜类、生物碱和类黄酮。抗菌试验表明，露天和室内培养的乙酸乙酯提取物在30mg/mL时对金黄色葡萄球菌表现出中等的活性，抑制区的值分别为13.3±0.67mm和11.0±0.58mm。

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Phytochemical Screening and Antibacterial Activity of Lignosus rhinocerotis (Cooke) Ryvarden Grown in Open Field and Indoor

Lignosus rhinocerotis (Cooke) Ryvarden, known as tiger milk mushroom, is a rare and valuable medicinal mushroom that is widely used throughout Southeast Asia and South China for treating several ailments. This study was conducted to screen the phytochemicals present in L. rhinocerotis (Cooke) Ryvarden sclerotium grown from two different environments, open field and indoor, and evaluate the antibacterial activity. In this study, phytochemical screening of ethyl acetate and methanolic extract of L. rhinocerotis (Cooke) Ryvarden sclerotium were done using various chemical tests to identify the compounds present in the extracts. All the extracts were then tested for antibacterial activity against three different bacteria including Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa at the concentrations of 20 and 30 mg/mL using disc diffusion method. Based on the phytochemical screening result, extracts of L. rhinocerotis (Cooke) Ryvarden demonstrated the presence of steroids, terpenoids, alkaloids, and flavonoids. The antibacterial assays revealed that the ethyl acetate extracts from open field and indoor cultivations exhibited moderate activities against S. aureus at 30 mg/mL with the values of the inhibition zone being 13.3 ± 0.67 mm and 11.0 ± 0.58 mm, respectively.

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来源期刊

gazi university journal of science MULTIDISCIPLINARY SCIENCES-

CiteScore

1.60

自引率

11.10%

发文量

期刊介绍： The scope of the “Gazi University Journal of Science” comprises such as original research on all aspects of basic science, engineering and technology. Original research results, scientific reviews and short communication notes in various fields of science and technology are considered for publication. The publication language of the journal is English. Manuscripts previously published in another journal are not accepted. Manuscripts with a suitable balance of practice and theory are preferred. A review article is expected to give in-depth information and satisfying evaluation of a specific scientific or technologic subject, supported with an extensive list of sources. Short communication notes prepared by researchers who would like to share the first outcomes of their on-going, original research work are welcome.