Anti-inflammatory activity of Lactobacillus spp. and Rhodopseudomonas palustris probiotics

Background: Probiotics have been used for many years to promote human health by mitigating  inflammation. However, its mechanics have not been fully elucidated. During inflammation, excessive and/or prolonged production of pro-inflammatory cytokines is related with various inflammatory diseases and cancer. Several probiotics have been reported as playing a role in suppressing the level of pro-inflammatory cytokines, as the human body attempts to recover. The aim of this study was to evaluate the anti-inflammatory activity of probiotics consisting of Lactobacillus spp. and Rhodopseudomonas palustris on macrophage RAW 264.7 cells.Methods: The probiotics mixture was centrifuged to separate supernatant, i.e., the probiotics extract, from the cells. The extract was then evaluated for its effects on cell viability and anti-inflammatory activity of LPS inflammation-induced RAW264.7 cells.Results: The results showed that the extract of the probiotics was able to decrease the levels of IFN-γ, IL-1β, TNF-α, IL-8, TGF-β1 pro-inflammatory cytokines/mRNAs, and increase the level of IL-10 anti-inflammatory mRNA.Conclusion: The probiotics extract was identified to have anti-inflammatory activity, as it decreased the level of pro-inflammatory cytokines and increased the level of anti-inflammatory cytokines/mRNAs.Keywords: multi-strain probiotics, anti-inflammatory cytokine/mRNA, RAW264.7