参芪养心汤对扩张型心肌病大鼠高迁移率组box 1及炎症信号通路的影响

IF 2 4区 医学 Q2 INTEGRATIVE & COMPLEMENTARY MEDICINE
Shen Lijuan , Lu Shu , Zhou Yonghua , Xing Qingmin , Zhou Chungang , Li Lan
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引用次数: 2

摘要

目的探讨参芪养心汤对扩张型心肌病(DCM)模型大鼠心功能的影响及其可能机制。方法将sd - dawley大鼠随机分为正常组(10只)和DCM组(150只)。腹腔注射阿霉素诱导DCM。然后,DCM基线组随机选取16只DCM大鼠。其余DCM大鼠随机分为DCM对照组、培哚普利组、美托洛尔组和七味散组。心功能、组织学分析及生化测定血清脑钠肽(BNP)、炎症因子水平。测定大鼠高迁移率组盒1 (HMGB1)、toll样受体4 (TLR-4)、晚期糖基化终产物受体(RAGE)和核因子-κB (NF-κB) mRNA和蛋白表达水平。对正常组、清饮组和DCM对照组进行心肌代谢显像,评价治疗效果。结果DCM对照组大鼠左室直径扩大,心功能受损,肌节紊乱,糖代谢异常,心重指数升高,脑钠肽水平升高,经七味散治疗后均有改善。此外,DCM基线组大鼠心脏HMGB1、TLR-4、RAGE、NF-κB、肿瘤坏死因子-α、白细胞介素-1、白细胞介素-6、白细胞介素-10水平明显高于正常组。有趣的是,DCM基线组HMGB1 mRNA水平与TLR-4、RAGE、NF-κB、BNP、LVEDD呈正相关,与LVEF呈负相关。SQYXD抑制HMGB1表达上调及其下游炎性因子。结论参芪养心汤能有效降低扩张型心肌病患者左室直径,改善心功能。其作用机制涉及调节DCM大鼠模型中HMGB1基因和蛋白表达及其炎症信号通路。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effect of Shenqi Yangxin decoction on high mobility group box 1 and inflammatory signal pathway in a rat model of dilated cardiomyopathy

OBJECTIVE

To investigate the effects of Shenqi Yangxin decoction (SQYXD) on heart function in a rat model of dilated cardiomyopathy (DCM) and its potential mechanisms.

METHODS

Sprague-Dawley rats were randomly divided into normal (10 rats) and DCM (150 rats) groups. DCM was induced by an intraperitoneal injection of adriamycin. Then, DCM baseline group was randomly selected sixteen DCM rats. The remaining DCM rats were randomly divided into DCM control, perindopril, metoprolol, and SQYXD groups. Cardiac function and histological analysis plus biochemical measurement of serum levels of brain natriuretic peptide (BNP), and inflammatory factors were measured. The mRNA and protein expression levels of high-mobility group box 1 (HMGB1), Toll-like receptor 4 (TLR-4), receptor for advanced glycation end products (RAGE), and nuclear factor-κB (NF-κB) were determined. Myocardial metabolism imaging was performed on the normal, SQYXD and DCM control groups to evaluate the effectiveness of treatments.

RESULTS

Rats in the DCM control group exhibited dilated left ventricular diameter, impaired cardiac function, disorganized sarcomere, impaired glucose metabolism, increased heart weight index, and increased levels of BNP, which were improved by treatment with SQYXD. In addition, hearts from rats in the DCM baseline group exhibited significantly higher levels of HMGB1, TLR-4, RAGE, NF-κB, tumor necrosis factor-α, interleukin-1, interleukin-6, interleukin-10, compared with the normal group. Interestingly, the mRNA level of HMGB1 in the DCM baseline group was positively correlated with that of TLR-4, RAGE, NF-κB, BNP, and LVEDD, but negatively correlated with LVEF. SQYXD inhibited the upregulation of HMGB1 expression and its downstream inflammatory factors.

CONCLUSION

Shenqi Yangxin decoction effectively reduced the dilated left ventricular diameter and improved heart function in dilated cardiomyopathy. The mechanisms underlying the action on DCM involve regulating the gene and protein expression of HMGB1 and its inflammatory signal pathways in the DCM rat model.

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来源期刊
Journal of Traditional Chinese Medicine
Journal of Traditional Chinese Medicine INTEGRATIVE & COMPLEMENTARY MEDICINE-
CiteScore
2.40
自引率
3.80%
发文量
32269
审稿时长
2 months
期刊介绍: Journal of Traditional Chinese Medicine(JTCM) is devoted to clinical and theortical research on the use of acupuncture and Oriental medicine. The main columns include Clinical Observations, Basic Investigations, Reviews, Questions and Answers, an Expert''s Forum, and Discussions of Clinical Cases. Its key topics include acupuncture and electro-acupuncture, herbal medicine, homeopathy, masseotherapy, mind-body therapies, palliative care, and other CAM therapies.
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