多发性原发性恶性肿瘤生长对BALB/c裸小鼠下丘脑-垂体-性腺轴的影响

Cardiometry Pub Date : 2023-05-04 DOI:10.18137/cardiometry.2023.27.2632

O. Kit, E. Frantsiyants, V. Bandovkina, I. Kaplieva, A. Shikhlyarova, E. Surikova, I. Neskubina, D. Cheryarina, Y. Pogorelova, L. K. Trepitaki, I. Kotieva, L. Rozenko, Marina A. Arzamastseva, E. Agarkova, M. Sergeeva, V. Irina, Neskubina, K. Lidia, Trepitaki, A. Marina, Arzamastseva

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引用次数: 0

摘要

目的是研究患有多种原发性恶性肿瘤（MPMT）的两性BALB/c裸鼠下丘脑-垂体-性腺（HPG）轴功能的变化。将两种性别的BALB/c裸鼠（n=84）分为以下组：完整的雄性和雌性（n=14），这是一组具有B16/F10黑色素瘤标准皮下接种的两种性别小鼠的参考组（n=14；具有MPMT模型繁殖的主要小鼠组（n＝14）；将B16/F10黑色素瘤和Lewis癌依次接种到小鼠的两侧皮下：一个在左侧，另一个在右侧。恶性肿瘤生长15天后，使用标准试剂盒通过ELISA在1%的下丘脑和垂体组织匀浆中测定GnRH、LH和FSH含量，在10%的卵巢和睾丸匀浆中测定雌二醇含量（E2），以及用标准RIA试剂盒（Immunotech，捷克共和国）鉴定血清中睾酮（T）和孕酮（P4）的浓度。后果在参考组和主要组的雌性下丘脑中，GnRH水平增加了2.5-4倍，雄性平均下降了3.1倍；在参考组和主要组中，雌性动物垂体中的LH水平增加了1.3-1.6倍，但所有组中FSH的下降在雌性动物中更为明显：减少了7.8-13.7倍，在雄性动物中不太明显：减少1.4-1.7倍（р0,05）。在雌性性腺中，E2水平升高，但T和P4水平降低，而P4水平升高。血液参数不能反映器官中的激素变化。结论HHG轴表现的异常，以及原发性免疫缺陷，在克服异常恶性肿瘤的抗肿瘤保护方面起着决定性作用。

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Effect made by the growth of multiple primary malignant tumors on the hypothalamicpituitary-gonadal axis in BALB/c Nude mice of both sexes

The aim was to study changes in the functioning of the hypothalamic-pituitary-gonadal (HPG) axis in BALB/c Nude mice of both sexes with multiple primary malignant tumors (MPMTs). The BALB/c Nude mice (n=84) of both sexes were divided into groups as follows: intact males and females (n=14), a reference group of mice of both sexes with standard subcutaneous inoculation of B16/F10 melanoma (n=14); the main group of mice with the reproduction of the MPMT model (n=14); B16/ F10 melanoma and Lewis carcinoma were inoculated into mice sequentially subcutaneously on two sides: one on the left side and the other on the right side. After 15 days of the growth of the malignant tumors, GnRH, LH and FSH contents were determined by ELISA using standard kits in 1% of the homogenates of the hypothalamic and pituitary tissues, and estradiol content (E2) was determined in 10% homogenates of ovaries and testes, as well concentrations of testosterone (T) and progesterone (P4) were identified in blood serum with the standard RIA kits (Immunotech, Czech Republic). Results. In the hypothalamus in females of the reference and main groups, the level of GnRH increased by 2.5-4 times, and in males it decreased by an average of 3.1 times; in response to those changes in animals of both sexes in the reference group and in the main group only in females in the pituitary gland the level of LH increased by 1.3-1.6 times, however a decrease in FSH in all groups was more pronounced in females: by 7.8-13.7 times and less pronounced in males: by 1.4-1.7 times (р˂0, 05). In the gonads of females, the level of E2 increased, but the levels of T and P4 decreased, while P4 increased in males. Blood parameters did not reflect hormonal changes in the organs. Conclusion. Abnormalities in the HHG axis performance, along with primary immunodeficiency, play a decisive role in overcoming antitumor protection for uncharacteristic malignant tumors.

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来源期刊

Cardiometry MEDICAL LABORATORY TECHNOLOGY-

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0.00%

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审稿时长

6 weeks

期刊介绍： Cardiometry is an open access biannual electronic journal founded in 2012. It refers to medicine, particularly to cardiology, as well as oncocardiology and allied science of biophysics and medical equipment engineering. We publish mainly high quality original articles, reports, case reports, reviews and lectures in the field of the theory of cardiovascular system functioning, principles of cardiometry, its diagnostic methods, cardiovascular system therapy from the aspect of cardiometry, system and particular approaches to maintaining health, engineering peculiarities in cardiometry developing. The interdisciplinary areas of the journal are: hemodynamics, biophysics, biochemistry, metrology. The target audience of our Journal covers healthcare providers including cardiologists and general practitioners, bioengineers, biophysics, medical equipment, especially cardiology diagnostics device, developers, educators, nurses, healthcare decision-makers, people with cardiovascular diseases, cardiology and engineering universities and schools, state and private clinics. Cardiometry is aimed to provide a wide forum for exchange of information and public discussion on above scientific issues for the mentioned experts.