兔肠上皮钙通道(TRPV6)的表达模式

IF 0.8 4区农林科学 Q3 AGRICULTURE, DAIRY & ANIMAL SCIENCE

World Rabbit Science Pub Date : 2020-12-30 DOI:10.4995/wrs.2020.12161

R. Ranjan, P. Das, S. Batabyal, A. P. Minj

{"title":"兔肠上皮钙通道(TRPV6)的表达模式","authors":"R. Ranjan, P. Das, S. Batabyal, A. P. Minj","doi":"10.4995/wrs.2020.12161","DOIUrl":null,"url":null,"abstract":"The present study was undertaken to explore the immunohistochemical localisation of TRPV6 calcium channels in rabbit gut epithelium that are actively involved in calcium absorption. To undertake the research, twelve apparently healthy adult female rabbits with a body weight between 1.0 to 1.5 kg were procured, acclimatised and divided into two groups: control and test. Both groups were kept on same feed along with exogenous calcium supplementation in test group animals only. The serum calcium level revealed that normally a high value of serum calcium is maintained in the rabbit as compared to other mammals, thus indicating that the homeostatic mechanism might be poorly developed. Immunohistochemistry and reverse transcription polymerase chain reaction analysis revealed that the caecum was the site of maximum calcium absorption in rabbit, followed by the duodenum and jejunum. The expression pattern of TRPV6 protein/mRNA was weaker in test group animals than in the control group, indicating that the channel was functional in low calcium concentration in the gut.","PeriodicalId":23902,"journal":{"name":"World Rabbit Science","volume":null,"pages":null},"PeriodicalIF":0.8000,"publicationDate":"2020-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Patterns of calcium channel (TRPV6) expression in rabbit gut epithelium\",\"authors\":\"R. Ranjan, P. Das, S. Batabyal, A. P. Minj\",\"doi\":\"10.4995/wrs.2020.12161\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The present study was undertaken to explore the immunohistochemical localisation of TRPV6 calcium channels in rabbit gut epithelium that are actively involved in calcium absorption. To undertake the research, twelve apparently healthy adult female rabbits with a body weight between 1.0 to 1.5 kg were procured, acclimatised and divided into two groups: control and test. Both groups were kept on same feed along with exogenous calcium supplementation in test group animals only. The serum calcium level revealed that normally a high value of serum calcium is maintained in the rabbit as compared to other mammals, thus indicating that the homeostatic mechanism might be poorly developed. Immunohistochemistry and reverse transcription polymerase chain reaction analysis revealed that the caecum was the site of maximum calcium absorption in rabbit, followed by the duodenum and jejunum. The expression pattern of TRPV6 protein/mRNA was weaker in test group animals than in the control group, indicating that the channel was functional in low calcium concentration in the gut.\",\"PeriodicalId\":23902,\"journal\":{\"name\":\"World Rabbit Science\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.8000,\"publicationDate\":\"2020-12-30\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"World Rabbit Science\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.4995/wrs.2020.12161\",\"RegionNum\":4,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"AGRICULTURE, DAIRY & ANIMAL SCIENCE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"World Rabbit Science","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.4995/wrs.2020.12161","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"AGRICULTURE, DAIRY & ANIMAL SCIENCE","Score":null,"Total":0}

引用次数: 0

摘要

本研究旨在探讨兔肠道上皮中积极参与钙吸收的TRPV6钙通道的免疫组织化学定位。试验选用12只体重在1.0 ~ 1.5 kg之间、表面健康的成年母兔，驯化后分为对照组和试验组。两组均饲喂相同饲料，仅试验组添加外源钙。血清钙水平显示，与其他哺乳动物相比，家兔的血清钙通常维持在较高的水平，从而表明体内平衡机制可能发育不全。免疫组织化学和逆转录聚合酶链反应分析显示，兔盲肠是钙吸收最多的部位，其次是十二指肠和空肠。实验组动物TRPV6蛋白/mRNA的表达模式较对照组弱，说明该通道在肠道低钙浓度条件下具有一定的功能。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Patterns of calcium channel (TRPV6) expression in rabbit gut epithelium

The present study was undertaken to explore the immunohistochemical localisation of TRPV6 calcium channels in rabbit gut epithelium that are actively involved in calcium absorption. To undertake the research, twelve apparently healthy adult female rabbits with a body weight between 1.0 to 1.5 kg were procured, acclimatised and divided into two groups: control and test. Both groups were kept on same feed along with exogenous calcium supplementation in test group animals only. The serum calcium level revealed that normally a high value of serum calcium is maintained in the rabbit as compared to other mammals, thus indicating that the homeostatic mechanism might be poorly developed. Immunohistochemistry and reverse transcription polymerase chain reaction analysis revealed that the caecum was the site of maximum calcium absorption in rabbit, followed by the duodenum and jejunum. The expression pattern of TRPV6 protein/mRNA was weaker in test group animals than in the control group, indicating that the channel was functional in low calcium concentration in the gut.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

World Rabbit Science 农林科学-奶制品与动物科学

CiteScore

1.70

自引率

25.00%

发文量

审稿时长

>36 weeks

期刊介绍： World Rabbit Science is the official journal of the World Rabbit Science Association (WRSA). One of the main objectives of the WRSA is to encourage communication and collaboration among individuals and organisations associated with rabbit production and rabbit science in general. Subject areas include breeding, genetics, production, management, environment, health, nutrition, physiology, reproduction, behaviour, welfare, immunology, molecular biology, metabolism, processing and products. World Rabbit Science is the only international peer-reviewed journal included in the ISI Thomson list dedicated to publish original research in the field of rabbit science. Papers or reviews of the literature submitted to World Rabbit Science must not have been published previously in an international refereed scientific journal. Previous presentations at a scientific meeting, field day reports or similar documents can be published in World Rabbit Science, but they will be also subjected to the peer-review process. World Rabbit Science will publish papers of international relevance including original research articles, descriptions of novel techniques, contemporaryreviews and meta-analyses. Short communications will only accepted in special cases where, in the Editor''s judgement, the contents are exceptionally exciting, novel or timely. Proceedings of rabbit scientific meetings and conference reports will be considered for special issues. World Rabbit Science is published in English four times a year in a single volume. Authors may publish in World Rabbit Science regardless of the membership in the World Rabbit Science Association, even if joining the WRSA is encouraged. Views expressed in papers published in World Rabbit Science represent the opinion of the author(s) and do not necessarily reflect the official policy of the WRSA or the Editor-in-Chief.