水牛αs1-酪蛋白基因的分子表征

IF 0.2 4区农林科学 Q4 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Buffalo Bulletin Pub Date : 2022-09-28 DOI:10.56825/bufbu.2022.4131234

B. K. Pramanik, S. Batabyal, A. Maity, S. De, S. Chattopadhyay, A. Barui

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引用次数: 0

摘要

酪蛋白（αs1、αs2、β和κ）构成反刍动物乳汁的主要蛋白质成分，并以稳定的磷酸钙胶束的形式分泌。本研究旨在从分子水平上鉴定水牛αs1酪蛋白基因，以确定完整的αs1酪蛋白cDNA序列。对测序结果进行分析，并与其他家畜的可用mRNA GenBank序列（登录号AAB34797、NM_001009795、X72221、AY344966、NM_181029、AY948385和BC109618）进行比对。从克隆的乳腺组织信使核糖核酸cDNA中推导出水牛αs1-酪蛋白的完整氨基酸编码区。氨基酸序列由199个氨基酸残基组成。当与先前报道的水牛序列（Accession-AY948385）比对时，其在氨基酸位置78处显示一个氨基酸（谷氨酰胺）缺失。

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Molecular characterization of buffalo αs1-casein gene

The caseins (Alpha s1, Alpha s2, beta and kappa) comprise the major protein component of ruminant milk and are secreted in the form of stable calcium phosphate micelles. The present study was proposed to characterize αs1-casein gene in buffalo at the molecular level to determine complete αs1-casein cDNA sequence. Sequencing results were analyzed and aligned with available mRNA GenBank sequences of other domestic animals (Accession AAB34797, NM_001009795, X72221, AY344966, NM_181029, AY948385 and BC109618). The complete amino acid coding region of the buffalo αs1- casein was deduced from cloned cDNA generated from mammary tissue mRNA. Amino acid sequence comprises of 199 amino acid residues. When aligned with previously reported buffalo sequence (Accession-AY948385) it showed one amino acid (Glutamine) deletion at amino acid position 78.

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来源期刊

Buffalo Bulletin AGRICULTURE, DAIRY & ANIMAL SCIENCE-

CiteScore

0.50

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Buffalo Bulletin is published quarterly in January-March, April-June, July-September and October-December. Contributions on any aspect of research or development, progress reports of projects and news on buffalo will be considered for publication in the bulletin.