具有淀粉样蛋白-β的金纳米粒子降低神经细胞的细胞毒性,用于阿尔茨海默病的治疗和护理

IF 2.2 4区 工程技术 Q2 Chemistry
Qing Hong, Xinchun Jin, Chenheng Zhou, Jiahui Shao
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引用次数: 0

摘要

蛋白质低聚会导致阿尔茨海默病的发展。可以加速蛋白质低聚的纳米粒子通常被认为是有害的。据报道,金纳米粒子(AuNPs)在生物平台上取得了进展,但它们也可能具有刺激蛋白质低聚的能力。我们的目的是研究在AuNPs存在下淀粉样蛋白-β1-42(Aβ1-42)的神经毒性和低聚作用。沉淀方法用于产生AuNP,然后使用透射电子显微镜(TEM)、ThT、刚果红和CD光谱对其进行分析。结果表明,50nm尺寸的AuNPs在Aβ1-42中引导加速度。此外,对PC12细胞的细胞毒性研究表明,在诱导细胞死亡、氧化性凋亡、应激和膜渗漏方面,具有AuNPs的Aβ1-42比未处理的低聚物Aβ1-41毒性更小。总之,我们的研究揭示了AuNPs如何通过与阿尔茨海默病中的蛋白质结合来刺激细胞毒性低聚物的发育。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Gold nanoparticles with amyloid-β reduce neurocell cytotoxicity for the treatment and care of Alzheimer’s disease therapy

Gold nanoparticles with amyloid-β reduce neurocell cytotoxicity for the treatment and care of Alzheimer’s disease therapy

Protein oligomerization contributes to Alzheimer’s disease development (AD). A nanoparticle that can speed up the oligomerization of proteins is generally considered harmful. Gold nanoparticles (AuNPs) have been reported to be making headway in biological platforms, but they may also have the capacity to stimulate protein oligomerization. Our goal herein was to investigate the neurotoxicity and oligomerization of amyloid-β-1-42 (Aβ1-42) in the presence of AuNPs. The precipitation approach was used to create AuNPs, which were then analyzed using transmission electron microscopy (TEM), ThT, Congo red, and CD spectroscopy. The results demonstrated that the 50-nm-sized fabricated AuNPs guided acceleration in Aβ1-42. In addition, cytotoxicity studies on PC 12 cells showed that Aβ1-42 with AuNPs were less toxic than untreated oligomers Aβ1-42 in terms of inducing cell death, oxidative apoptosis, stress, and membrane leakage. In conclusion, our investigation sheds light on how AuNPs stimulate the development of cytotoxic oligomers by binding to proteins in Alzheimer’s disease.

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来源期刊
Gold Bulletin
Gold Bulletin 工程技术-材料科学:综合
CiteScore
3.30
自引率
4.50%
发文量
0
审稿时长
3 months
期刊介绍: Gold Bulletin is the premier international peer reviewed journal on the latest science, technology and applications of gold. It includes papers on the latest research advances, state-of-the-art reviews, conference reports, book reviews and highlights of patents and scientific literature. Gold Bulletin does not publish manuscripts covering the snthesis of Gold nanoparticles in the presence of plant extracts or other nature-derived extracts. Gold Bulletin has been published over 40 years as a multidisciplinary journal read by chemists, physicists, engineers, metallurgists, materials scientists, biotechnologists, surface scientists, and nanotechnologists amongst others, both within industry and academia. Gold Bulletin is published in Association with the World Gold Council.
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