转座子介导转基因蚊子的产生和验证。

Cold Spring Harbor protocols Pub Date : 2024-10-01 DOI:10.1101/pdb.prot108194

Vanessa Bottino-Rojas, Anthony A James

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引用次数: 0

摘要

转座子介导的转基因已经彻底改变了蚊子疾病媒介的基础和应用研究。目前，增强子陷阱和转座子标记等技术依赖于可再活化的插入突变，只有基于转座子的载体系统才有可能实现。在这里，我们提供了基于转座子的蚊子转基因的方法和应用的一般描述。确切的程序必须适用于每种蚊子，并概述了不同蚊子物种之间的一些差异的比较。许多优秀的出版物展示了详细和具体的协议和方法，并提供了特色和参考。

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Generating and Validating Transgenic Mosquitoes with Transposon-Mediated Transgenesis.

Transposon-mediated transgenesis has revolutionized both basic and applied studies of mosquito vectors of disease. Currently, techniques such as enhancer traps and transposon tagging, which rely on remobilizable insertional mutagenesis, are only possible with transposon-based vector systems. Here, we provide general descriptions of methods and applications of transposon-based mosquito transgenesis. The exact procedures must be adapted to each mosquito species and comparisons of some differences among different mosquito species are outlined. A number of excellent publications showing detailed and specific protocols and methods are featured and referenced.

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来源期刊

Cold Spring Harbor protocols Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)

CiteScore

3.00

自引率

0.00%

发文量

163

期刊介绍： Cold Spring Harbor Laboratory is renowned for its teaching of biomedical research techniques. For decades, participants in its celebrated, hands-on courses and users of its laboratory manuals have gained access to the most authoritative and reliable methods in molecular and cellular biology. Now that access has moved online. Cold Spring Harbor Protocols is an interdisciplinary journal providing a definitive source of research methods in cell, developmental and molecular biology, genetics, bioinformatics, protein science, computational biology, immunology, neuroscience and imaging. Each monthly issue details multiple essential methods—a mix of cutting-edge and well-established techniques.