Scribble, Lgl1和myosin IIA与α-/β-连环蛋白相互作用以维持上皮连接的完整性。

IF 3.3 3区 生物学 Q3 CELL BIOLOGY
Cell Adhesion & Migration Pub Date : 2023-12-01 Epub Date: 2023-09-24 DOI:10.1080/19336918.2023.2260645
Maha Abedrabbo, Shirel Sloomy, Reham Abu-Leil, Einav Kfir-Cohen, Shoshana Ravid
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引用次数: 0

摘要

E-钙粘蛋白-连环蛋白复合物与细胞骨架一起构建粘附连接(AJs)的核心。据报道,Scribble稳定E-钙粘蛋白与连环蛋白的偶联,促进上皮细胞粘附,但其机制尚不清楚。我们发现Scrible、Lgl1和NMII-A与E-钙粘蛋白-连环蛋白复合物存在于一个复合物中。Scrible或Lgl1的缺失破坏了E-钙粘蛋白-连环蛋白复合物在AJ中的定位。Lgl1的aPKCζ磷酸化调节Lgl1和E-钙粘蛋白-连环蛋白复合物的AJ定位。Scrible和Lgl1都调节AJ处NMII-A的激活和募集。最后,Scrible和Lgl1被TGFβ诱导的EMT下调,并且它们在EMT过程中的重新表达阻碍了其进展。我们的研究结果深入了解了Scrible、Lgl1和NMII-A调节AJ完整性的机制。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Scribble, Lgl1, and myosin IIA interact with α-/β-catenin to maintain epithelial junction integrity.

E-cadherin-catenin complex together with the cytoskeleton, builds the core of Adherens junctions (AJs). It has been reported that Scribble stabilizes the coupling of E-cadherin with catenins promoting epithelial cell adhesion, but the mechanism remains unknown. We show that Scribble, Lgl1, and NMII-A reside in a complex with E-cadherin-catenin complex. Depletion of either Scribble or Lgl1 disrupts the localization of E-cadherin-catenin complex to AJs. aPKCζ phosphorylation of Lgl1 regulates AJ localization of Lgl1 and E-cadherin-catenin complexes. Both Scribble and Lgl1 regulate the activation and recruitment of NMII-A at AJs. Finally, Scribble and Lgl1 are downregulated by TGFβ-induced EMT, and their re-expression during EMT impedes its progression. Our results provide insight into the mechanism regulating AJ integrity by Scribble, Lgl1, and NMII-A.

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来源期刊
CiteScore
6.40
自引率
0.00%
发文量
7
审稿时长
53 weeks
期刊介绍: Cell Adhesion & Migration is a multi-disciplinary, peer reviewed open access journal that focuses on the biological or pathological implications of cell-cell and cell-microenvironment interactions. The main focus of this journal is fundamental science. The journal strives to serve a broad readership by regularly publishing review articles covering specific disciplines within the field, and by publishing focused issues that provide an overview on specific topics of interest within the field. Cell Adhesion & Migration publishes relevant and timely original research, as well as authoritative overviews, commentaries, and perspectives, providing context for the work presented in Cell Adhesion & Migration and for key results published elsewhere. Original research papers may cover all topics important in the field of cell-cell and cell-matrix interactions. Cell Adhesion & Migration also publishes articles related to cell biomechanics, biomaterial, and development of related imaging technologies.
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