{"title":"木霉TM11对蓝莓根腐病的防治作用及机理。","authors":"Si Li, Fu-Mei Zhang, Xiao-Jing Shang, Rui Hou","doi":"10.33073/pjm-2023-034","DOIUrl":null,"url":null,"abstract":"<p><p><i>Fusarium oxysporum</i> is the primary pathogen of blueberry root rot; furthermore, we found that <i>Fusarium commune</i> can also cause root rot in blueberries. <i>Trichoderma</i> spp. is widely used to control plant diseases. We isolated <i>Trichoderma asperellum</i> (TM11) from blueberry rhizosphere soil to explore its control effect and mechanism on <i>F. oxysporum</i> and <i>F. commune</i>. We found that the inhibitory effects of TM11 volatiles and broth metabolites on <i>F. oxysporum</i> were significant, but only <i>F. commune</i> volatile metabolites had a significant inhibitory effect on its growth. Twelve known antimicrobial metabolites were detected from the methanol extract of TM11 fermentation broth by HPLC-MS. TM11 lysed and coiled around the hyphae of <i>F. oxysporum</i> and <i>F. commune</i>. The pot experiment showed that TM11 had significant control effects against <i>F. oxysporum</i> and <i>F. commune</i>, and inoculation of TM11 prior to that of <i>F. oxysporum</i> and <i>F. commune</i> was more effective. The TM11, TM11 and <i>F. oxysporum</i>, or <i>F. commune</i> and distilled water treatments had different effects on the activities of superoxide dismutase, peroxidase and catalase, and the enzyme activity levels exhibited the following order: TM11 > TM11 and <i>F. oxysporum</i> or <i>F. commune</i> > distilled water. The results showed that TM11 provided effective control of blueberry root rot.</p>","PeriodicalId":94173,"journal":{"name":"Polish journal of microbiology","volume":"72 3","pages":"325-337"},"PeriodicalIF":0.0000,"publicationDate":"2023-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/70/7a/pjm-72-3-pjm-2023-034.PMC10561078.pdf","citationCount":"0","resultStr":"{\"title\":\"Control Effect and Mechanism of <i>Trichoderma asperellum</i> TM11 against Blueberry Root Rot.\",\"authors\":\"Si Li, Fu-Mei Zhang, Xiao-Jing Shang, Rui Hou\",\"doi\":\"10.33073/pjm-2023-034\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p><i>Fusarium oxysporum</i> is the primary pathogen of blueberry root rot; furthermore, we found that <i>Fusarium commune</i> can also cause root rot in blueberries. <i>Trichoderma</i> spp. is widely used to control plant diseases. We isolated <i>Trichoderma asperellum</i> (TM11) from blueberry rhizosphere soil to explore its control effect and mechanism on <i>F. oxysporum</i> and <i>F. commune</i>. We found that the inhibitory effects of TM11 volatiles and broth metabolites on <i>F. oxysporum</i> were significant, but only <i>F. commune</i> volatile metabolites had a significant inhibitory effect on its growth. Twelve known antimicrobial metabolites were detected from the methanol extract of TM11 fermentation broth by HPLC-MS. TM11 lysed and coiled around the hyphae of <i>F. oxysporum</i> and <i>F. commune</i>. The pot experiment showed that TM11 had significant control effects against <i>F. oxysporum</i> and <i>F. commune</i>, and inoculation of TM11 prior to that of <i>F. oxysporum</i> and <i>F. commune</i> was more effective. The TM11, TM11 and <i>F. oxysporum</i>, or <i>F. commune</i> and distilled water treatments had different effects on the activities of superoxide dismutase, peroxidase and catalase, and the enzyme activity levels exhibited the following order: TM11 > TM11 and <i>F. oxysporum</i> or <i>F. commune</i> > distilled water. The results showed that TM11 provided effective control of blueberry root rot.</p>\",\"PeriodicalId\":94173,\"journal\":{\"name\":\"Polish journal of microbiology\",\"volume\":\"72 3\",\"pages\":\"325-337\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-09-20\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/70/7a/pjm-72-3-pjm-2023-034.PMC10561078.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Polish journal of microbiology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.33073/pjm-2023-034\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2023/9/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Polish journal of microbiology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.33073/pjm-2023-034","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2023/9/1 0:00:00","PubModel":"eCollection","JCR":"","JCRName":"","Score":null,"Total":0}
Control Effect and Mechanism of Trichoderma asperellum TM11 against Blueberry Root Rot.
Fusarium oxysporum is the primary pathogen of blueberry root rot; furthermore, we found that Fusarium commune can also cause root rot in blueberries. Trichoderma spp. is widely used to control plant diseases. We isolated Trichoderma asperellum (TM11) from blueberry rhizosphere soil to explore its control effect and mechanism on F. oxysporum and F. commune. We found that the inhibitory effects of TM11 volatiles and broth metabolites on F. oxysporum were significant, but only F. commune volatile metabolites had a significant inhibitory effect on its growth. Twelve known antimicrobial metabolites were detected from the methanol extract of TM11 fermentation broth by HPLC-MS. TM11 lysed and coiled around the hyphae of F. oxysporum and F. commune. The pot experiment showed that TM11 had significant control effects against F. oxysporum and F. commune, and inoculation of TM11 prior to that of F. oxysporum and F. commune was more effective. The TM11, TM11 and F. oxysporum, or F. commune and distilled water treatments had different effects on the activities of superoxide dismutase, peroxidase and catalase, and the enzyme activity levels exhibited the following order: TM11 > TM11 and F. oxysporum or F. commune > distilled water. The results showed that TM11 provided effective control of blueberry root rot.