木霉TM11对蓝莓根腐病的防治作用及机理。

Polish journal of microbiology Pub Date : 2023-09-20 eCollection Date: 2023-09-01 DOI:10.33073/pjm-2023-034
Si Li, Fu-Mei Zhang, Xiao-Jing Shang, Rui Hou
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引用次数: 0

摘要

尖孢镰刀菌是蓝莓根腐病的主要病原菌;此外,我们发现镰刀菌群落也会引起蓝莓的根腐病。木霉被广泛用于控制植物病害。从蓝莓根际土壤中分离得到粗糙木霉TM11,探讨其对尖孢镰刀菌和群落镰刀菌的防治作用及其机理。我们发现TM11挥发物和肉汤代谢产物对尖孢镰刀菌的生长有显著的抑制作用,但只有公社挥发物代谢产物对其生长有显著抑制作用。采用HPLC-MS法从TM11发酵液的甲醇提取物中检测到12种已知的抗菌代谢产物。TM11裂解并盘绕在尖孢镰刀菌和普通镰刀菌的菌丝上。盆栽试验表明,TM11对尖孢镰刀菌和普通镰刀菌有显著的防治效果,TM11的接种效果优于尖孢镰刀菌和普通菌株。TM11、TM11和尖孢镰刀菌,或F.community和蒸馏水处理对超氧化物歧化酶、过氧化物酶和过氧化氢酶活性的影响不同,酶活性水平依次为:TM11>TM11和尖孢镰刀豆或F.community>蒸馏水。结果表明,TM11能有效地控制蓝莓根腐病的发生。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Control Effect and Mechanism of Trichoderma asperellum TM11 against Blueberry Root Rot.

Fusarium oxysporum is the primary pathogen of blueberry root rot; furthermore, we found that Fusarium commune can also cause root rot in blueberries. Trichoderma spp. is widely used to control plant diseases. We isolated Trichoderma asperellum (TM11) from blueberry rhizosphere soil to explore its control effect and mechanism on F. oxysporum and F. commune. We found that the inhibitory effects of TM11 volatiles and broth metabolites on F. oxysporum were significant, but only F. commune volatile metabolites had a significant inhibitory effect on its growth. Twelve known antimicrobial metabolites were detected from the methanol extract of TM11 fermentation broth by HPLC-MS. TM11 lysed and coiled around the hyphae of F. oxysporum and F. commune. The pot experiment showed that TM11 had significant control effects against F. oxysporum and F. commune, and inoculation of TM11 prior to that of F. oxysporum and F. commune was more effective. The TM11, TM11 and F. oxysporum, or F. commune and distilled water treatments had different effects on the activities of superoxide dismutase, peroxidase and catalase, and the enzyme activity levels exhibited the following order: TM11 > TM11 and F. oxysporum or F. commune > distilled water. The results showed that TM11 provided effective control of blueberry root rot.

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